Laboratoire de Recherche sur les Lipoprotéines et l'Athérosclérose, Unité Associée au CNRST-URAC 34, Université Hassan II, Faculté des Sciences Ben M'Sik, Casablanca, Morocco.
Clin Biochem. 2012 Apr;45(6):470-4. doi: 10.1016/j.clinbiochem.2012.01.005. Epub 2012 Jan 18.
Paraoxonase 1 (PON1) is mainly complexed to HDL and is responsible, at least in part, for their antioxidant properties. The aims of our study were to determine the phenotype distribution and enzymatic activities of PON1 and the oxidative stress status of healthy subjects and diabetic and hemodialysis patients.
PON1 paraoxonase and arylesterase activities and oxidative stress markers [malondialdehyde (MDA) and vitamin E levels] were measured in 300 individuals as a function of health status.
The prevalence of the PON1 phenotypes in the study population was 74.51%, 18.15% and 7.34% for QQ, QR and RR, respectively. The phenotype distribution did not change significantly as a function of health status (healthy, diabetes, hemodialysis). However, the hemodialysis patients had lower PON1 paraoxonase and arylesterase activities than the diabetic patients and healthy subjects, while there were no significant differences between the diabetic patients and the healthy subjects. Oxidative stress markers (MDA levels and vitamin E/cholesterol ratio) were significantly higher in the diabetic and hemodialysis patients than in the healthy subjects.
The lower plasma PON1 enzymatic activities in the hemodialysis patients was not associated with a difference in the phenotype distribution of PON1. Oxidative stress conditions were significantly higher in these patients, which may increase the risk of atherosclerosis in this population.
对氧磷酶 1（PON1）主要与高密度脂蛋白（HDL）结合，并负责其抗氧化特性，至少部分负责。我们研究的目的是确定健康受试者、糖尿病和血液透析患者的 PON1 表型分布和酶活性以及氧化应激状态。
根据健康状况，测量了 300 个人的 PON1 对氧磷酶和芳基酯酶活性以及氧化应激标志物[丙二醛（MDA）和维生素 E 水平]。
在研究人群中，PON1 表型的患病率分别为 74.51%、18.15%和 7.34%，分别为 QQ、QR 和 RR。表型分布随健康状况（健康、糖尿病、血液透析）无明显变化。然而，血液透析患者的 PON1 对氧磷酶和芳基酯酶活性低于糖尿病患者和健康受试者，而糖尿病患者和健康受试者之间无显著差异。糖尿病和血液透析患者的氧化应激标志物（MDA 水平和维生素 E/胆固醇比值）明显高于健康受试者。
血液透析患者血浆 PON1 酶活性较低与 PON1 表型分布无差异有关。这些患者的氧化应激状态明显升高，这可能会增加该人群患动脉粥样硬化的风险。
