Hua Kun, Wang Xiao-wei, Zhang Wei, Zhou Jian, Yang Xiu-bin, Ji Jun-hui
Department of Surgery, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul;30(7):1501-4, 1508.
To evaluate biocompatibility and cytotoxicity of PBS/PLA blend as the material for sternal fixation.
L929 cell line was used to assess the cytotoxicity and cytocompatibility of PBS/PLA blend by incubation of the cells with the aqueous extract of the material for 3 days. The cytocompatibility of the material was evaluated by observing the proliferation of the cells cultured on the surface of the material. The proliferation of the cells cultured on the surface of the material was compared with that of the cells cultured on medical polyethylene and polyvinyl chloride to assess the cytotoxicity of the material.
L929 cells showed no morphological changes after a 3-day incubation with the aqueous extract of the material. The cells cultured on the surface of the blend material exhibited better proliferative activity than those cultured on medical polyethylene and polyvinyl chloride, demonstrating no obvious cytotoxicity of the material.
PBS/PLA blend material possesses excellent cytocompatibility and can be a promising biodegradable polymer for sternal fixation.
评估聚丁二酸丁二醇酯/聚乳酸（PBS/PLA）共混物作为胸骨固定材料的生物相容性和细胞毒性。
通过将L929细胞系与材料的水提取物孵育3天，来评估PBS/PLA共混物的细胞毒性和细胞相容性。通过观察在材料表面培养的细胞增殖情况来评估材料的细胞相容性。将在材料表面培养的细胞增殖情况与在医用聚乙烯和聚氯乙烯上培养的细胞进行比较，以评估材料的细胞毒性。
L929细胞与材料的水提取物孵育3天后未出现形态变化。在共混材料表面培养的细胞比在医用聚乙烯和聚氯乙烯上培养的细胞表现出更好的增殖活性，表明该材料无明显细胞毒性。
PBS/PLA共混材料具有优异的细胞相容性，可成为一种有前景的用于胸骨固定的可生物降解聚合物。
