Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Nat Methods. 2012 Jan 29;9(3):263-5. doi: 10.1038/nmeth.1867.
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.
基于 X 射线自由电子激光（X-FEL）的连续飞秒晶体学是一种新兴的方法，具有快速推进膜蛋白结构生物学这一具有挑战性领域的潜力。在这里，我们使用海绵相微喷射器将 Blastochloris viridis 光合作用反应中心的微米级脂质海绵相晶体递送到 X-FEL 光束中，记录到可解释的衍射数据。
