Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
Nat Methods. 2012 Jan 29;9(3):259-62. doi: 10.1038/nmeth.1859.
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
在自然界中已经观察到多次细胞内蛋白质结晶。然而,由于这些晶体体积小,尚未用于 X 射线晶体学分析。我们制备了纳米级的非洲锥虫酶的体内生长晶体,并应用新兴的基于自由电子激光的连续飞秒晶体学方法来记录可解释的衍射数据。这种组合方法将为结构系统生物学开辟新的机会。