Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 Nov;62(Pt 11):2750-2756. doi: 10.1099/ijs.0.039057-0. Epub 2012 Jan 27.
A Gram-negative, coccoid-shaped bacterium, strain CC-CCM15-8(T), was isolated from a rhizosphere soil sample of the plant Crossostephium chinense (L.) Makino (Seremban) from Budai Township, Chiayi County, Taiwan. 16S rRNA gene sequence analysis clearly allocated strain CC-CCM15-8(T) to the Paracoccus cluster, showing highest similarities to the type strains of 'Paracoccus beibuensis' (98.8%), Paracoccus homiensis (97.6%), Paracoccus aestuarii (97.7%) and Paracoccus zeaxanthinifaciens (97.7%). The fatty acid profile, comprising C(18:1)ω7c as the major component and C(10:0) 3-OH as the characteristic hydroxylated fatty acid, supported the placement of strain CC-CCM15-8(T) within the genus Paracoccus. The polyamine pattern consisted of putrescine and spermidine as major components. Ubiqinone Q-10 was the major quinone type (95%); ubiquinone Q-9 was also detected (5%). The complex polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, and unidentified phospholipids, lipids and glycolipids. Levels of DNA-DNA relatedness between strain CC-CCM15-8(T) and 'P. beibuensis' LMG 25871(T), P. aestuarii DSM 19484(T), P. zeaxanthinifaciens LMG 21993(T) and P. homiensis KACC 11518(T) were 24.9% (34.8%, reciprocal analysis), 15.7% (17.5%), 17.7% (23.4%) and 16.0% (25.4%), respectively. Physiological and biochemical test results allowed the phenotypic differentiation of strain CC-CCM15-8(T) from its closest relatives in the genus Paracoccus. Based on the data presented, it is concluded that strain CC-CCM15-8(T) represents a novel species of the genus Paracoccus, for which the name Paracoccus rhizosphaerae sp. nov. is proposed. The type strain is CC-CCM15-8(T) (=LMG 26205(T)=CCM 7904(T)).
一株革兰氏阴性、球形的细菌，菌株 CC-CCM15-8(T)，从台湾嘉义县布袋镇的植物 Crossostephium chinense (L.) Makino (Seremban) 的根际土壤样本中分离得到。16S rRNA 基因序列分析清楚地将菌株 CC-CCM15-8(T)归入 Paracoccus 群，与“Paracoccus beibuensis”（98.8%）、Paracoccus homiensis（97.6%）、Paracoccus aestuarii（97.7%）和 Paracoccus zeaxanthinifaciens（97.7%）的模式菌株最为相似。脂肪酸组成，主要成分为 C(18:1)ω7c，特征性的羟基脂肪酸为 C(10:0) 3-OH，这支持菌株 CC-CCM15-8(T) 归属于 Paracoccus 属。聚胺模式由腐胺和亚精胺作为主要成分组成。泛醌 Q-10 是主要的醌型（95%）；也检测到泛醌 Q-9（5%）。复杂的极性脂谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱和未鉴定的磷脂、脂类和糖脂组成。菌株 CC-CCM15-8(T)与“P. beibuensis”LMG 25871(T)、P. aestuarii DSM 19484(T)、P. zeaxanthinifaciens LMG 21993(T)和 P. homiensis KACC 11518(T)之间的 DNA-DNA 相关度分别为 24.9%（34.8%，相互分析）、15.7%（17.5%）、17.7%（23.4%）和 16.0%（25.4%）。生理生化试验结果表明，菌株 CC-CCM15-8(T)与其在 Paracoccus 属中最接近的亲缘关系可以通过表型进行区分。基于所提出的数据，结论是菌株 CC-CCM15-8(T)代表 Paracoccus 属的一个新种，提议将其命名为 Paracoccus rhizosphaerae sp. nov.。模式菌株为 CC-CCM15-8(T)=LMG 26205(T)=CCM 7904(T)。
