Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2011 May;61(Pt 5):1138-1143. doi: 10.1099/ijs.0.023333-0. Epub 2010 Jun 11.
A bacterial strain, designated SW-3(T), was isolated from the reef-building coral Isopora palifera, from Southern Taiwan, and was characterized by using a polyphasic taxonomic approach. Cells of strain SW-3(T) were Gram-reaction-negative, aerobic, rod-shaped and motile by means of monopolar flagella and formed cream-white colonies. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-3(T) belonged to the genus Paracoccus and its most closely related neighbours were P. aestuarii B7(T), P. homiensis DD-R11(T), P. marinus KKL-A5(T), P. denitrificans DSM 413(T) and P. zeaxanthinifaciens R-1512(T), with sequence similarities of 96.8, 96.6, 96.1, 95.8 and 94.9 %, respectively. Strain SW-3(T) exhibited optimal growth at 25-30 °C and pH 9-10 and in 3-5 % (w/v) NaCl. The predominant cellular fatty acid was summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c; 75 %). The major respiratory quinone was Q-10. The polar lipid profile consisted of a mixture of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and several uncharacterized polar lipids. The DNA G+C content of strain SW-3(T) was 63.7 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from recognized species of the genus Paracoccus. It is evident from the genotypic, phenotypic and chemotaxonomic data that strain SW-3(T) represents a novel species of the genus Paracoccus, for which the name Paracoccus isoporae sp. nov. is proposed. The type strain is SW-3(T) ( = LMG 25205(T) = BCRC 17967(T)).
一株细菌菌株，命名为 SW-3(T)，从台湾南部的造礁珊瑚 Isopora palifera 中分离得到，并通过多相分类方法进行了特征描述。SW-3(T)菌株的细胞革兰氏反应阴性，需氧，杆状，通过单极鞭毛运动，形成奶油色菌落。基于 16S rRNA 基因序列的系统发育分析表明，SW-3(T)菌株属于副球菌属，与其最密切相关的近缘种为 P. aestuarii B7(T)、P. homiensis DD-R11(T)、P. marinus KKL-A5(T)、P. denitrificans DSM 413(T)和 P. zeaxanthinifaciens R-1512(T)，其序列相似度分别为 96.8%、96.6%、96.1%、95.8%和 94.9%。SW-3(T)菌株在 25-30°C 和 pH 9-10 以及 3-5%（w/v）NaCl 下生长最佳。主要细胞脂肪酸为 summed feature 8 (C(18 : 1)ω7c 和/或 C(18 : 1)ω6c; 75%)。主要呼吸醌为 Q-10。极性脂谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱和几种未鉴定的极性脂组成。SW-3(T)菌株的 DNA G+C 含量为 63.7 mol%。生理生化测试的结果允许清楚地区分该分离株与副球菌属的公认种。从基因型、表型和化学分类学数据来看，SW-3(T)菌株代表副球菌属的一个新种，因此建议将其命名为 Paracoccus isoporae sp. nov.。模式菌株为 SW-3(T)（=LMG 25205(T)=BCRC 17967(T)）。
