Coburn Jeannine, Gibson Matt, Bandalini Pierre Alain, Laird Christopher, Mao Hai-Quan, Moroni Lorenzo, Seliktar Dror, Elisseeff Jennifer
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland.
Smart Struct Syst. 2011 Jan 1;7(3):213-222. doi: 10.12989/sss.2011.7.3.213.
The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.
天然细胞外基质（ECM）由一个整合的纤维蛋白网络和基于蛋白聚糖的基质（水凝胶）物质组成。我们设计了一种新型静电纺丝技术，以构建一种三维纤维-水凝胶复合材料，该材料模仿天然ECM结构，可注射，并且对于临床相关组织缺损具有实用的宏观尺寸。在关节软骨组织工程的模型系统中，纤维-水凝胶复合材料增强了成体干细胞的生物学反应，动态机械刺激导致细胞外基质接近天然水平。通过对纤维进行结构和生化修饰扩展了该技术平台，这些修饰包括含有硫酸软骨素（内源性组织的重要成分）的亲水性纤维以及含有ECM微粒的疏水性纤维。
