Li Boyangzi, Mock Feiyan, Wu Peng
Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA.
Methods Enzymol. 2012;505:401-19. doi: 10.1016/B978-0-12-388448-0.00029-2.
The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems.
细胞产生的聚糖的完整集合——糖组，是分子成像的一个有吸引力的靶点。最近，通过基于生物正交化学报告基团的方法，实现了对活体细胞中糖组的成像。在本章中，我们分别描述了两种将生物正交化学报告基团（标签）引入细胞表面岩藻糖基化聚糖和带有乳糖胺二糖的聚糖的方法。然后，这些带有标签的聚糖可以通过生物正交点击化学与成像探针结合。类似的方法可以扩展到对活体细胞中糖组的其他部分进行成像。
