Huang Zhen, Lippard Stephen J
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Methods Enzymol. 2012;505:445-68. doi: 10.1016/B978-0-12-388448-0.00031-0.
With the aid of chemoselective sensors, fluorescence microscopy has emerged as an indispensable tool to visualize the distribution and dynamics of various biologically important molecules in live specimens. Motivated by our interest in understanding the chemistry and biology of mobile zinc underlying its physiological and pathological roles, over the past decade, our laboratory has developed an extensive library of zinc fluorescence probes. In this chapter, we provide essential information about our sensor toolbox in order to assist investigators interested to apply our constructs to study various aspects of mobile zinc biology. We illustrate their use with several examples of imaging both exogenous and endogenous mobile zinc in live cells and tissues using various versions of fluorescence microscopy, including confocal and two-photon microscopy.
借助化学选择性传感器，荧光显微镜已成为可视化活标本中各种生物重要分子的分布和动态的不可或缺的工具。出于我们对理解移动锌在其生理和病理作用背后的化学和生物学的兴趣，在过去十年中，我们实验室开发了大量的锌荧光探针库。在本章中，我们提供了有关我们的传感器工具箱的基本信息，以帮助有兴趣应用我们的构建体来研究移动锌生物学各个方面的研究人员。我们通过使用各种版本的荧光显微镜（包括共聚焦显微镜和双光子显微镜）对活细胞和组织中的外源性和内源性移动锌进行成像的几个例子来说明它们的用途。
