Geng Y J, Xie S L, Li Q, Ma J, Wang G Y
Department of General Surgery, The First Clinical Hospital of Jilin University, Changchun, China.
J Int Med Res. 2011;39(6):2119-28. doi: 10.1177/147323001103900608.
Increasing evidence suggests that large intervening non-coding RNAs (lincRNAs) regulate key pathways in cancer invasion and metastasis. In this observational retrospective study, the expression of the oncogenic lincRNA HOX transcript antisense RNA (HOTAIR) gene was measured in 63 patients with hepatocellular carcinoma (HCC) following hepatic resection. The HOTAIR gene was significantly overexpressed in HCC tissues compared with adjacent non-tumour tissues. Patients with high HOTAIR gene expression in their tumours had an increased risk of recurrence after hepatectomy. There was also a significant correlation between HOTAIR expression and lymph node metastasis. In vitro assays in the HCC cell line Bel7402 demonstrated that knockdown of HOTAIR lincRNA reduced cell proliferation and was associated with reductions in levels of matrix metalloproteinase-9 and vascular endothelial growth factor protein, which are important for cell motility and metastasis. In conclusion, HOTAIR lincRNA might be a potential biomarker for the existence of lymph node metastasis in HCC.
越来越多的证据表明，长链非编码RNA（lincRNA）调控癌症侵袭和转移的关键途径。在这项观察性回顾性研究中，测定了63例肝癌（HCC）患者肝切除术后致癌性lincRNA HOX转录本反义RNA（HOTAIR）基因的表达。与癌旁非肿瘤组织相比，HOTAIR基因在HCC组织中显著过表达。肿瘤中HOTAIR基因高表达的患者肝切除术后复发风险增加。HOTAIR表达与淋巴结转移之间也存在显著相关性。在HCC细胞系Bel7402中的体外试验表明，敲低HOTAIR lincRNA可降低细胞增殖，并与基质金属蛋白酶-9和血管内皮生长因子蛋白水平降低相关，这两种蛋白对细胞运动和转移很重要。总之，HOTAIR lincRNA可能是HCC中存在淋巴结转移的潜在生物标志物。
