Department of Microbiology and Clinical Microbiology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Indian J Pediatr. 2013 Feb;80(2):91-6. doi: 10.1007/s12098-012-0880-z. Epub 2012 Sep 29.
To determine the frequency rate of C. pneumoniae, rhinovirus, respiratory syncytial virus (RSV), influenza virus, metapneumovirus, adenovirus', parainfluenza virus and coronavirus in acute respiratory tract infections in children.
One hundred nine pediatric patients having respiratory tract infections were included in this study. Real time PCR, DFA and cell culture method were used for detection of C. pneumoniae, RSV antigen and influenza virus respectively. Multiplex PCR was used for detection of other viruses.
No C. pneumoniae DNA was detected in the samples. Virus was detected in 43 cases from larynx swabs (43/109, 39.4 %). The frequency order of the viral agents detected were as follows; rhinoviruses 14.7 %, RSV B 7.3 %, influenza A 6.4 %, metapneumovirus 3.6 %, adenovirus 3.6 %, coronavirus 0.9 %, parainfluenzavirus type 3, 0.9 %, parainfluenzavirus type 4, 0.9 % and RSV A 0.9 %. Sensitivity of the PCR and DFA methods for the diagnosis of RSV infections were detected as 100 % and 100 %, respectively. Specificity of the PCR and DFA methods for RSV infections were detected as 97 % and 100 % respectively. Sensitivity of the PCR and cell culture methods for influenzavirus infections were detected as 100 % and 100 %, respectively. Specificity of the PCR and DFA methods for RSV infections were detected as 96 % and 100 % respectively.
Prevalence of viral agents was detected as 39.4 %. Influenza viruses and RSV were common. Metapneumovirus was also frequent (3.6 %). C. pneumoniae was not found to be a common agent for acute respiratory disease in children.
确定肺炎衣原体、鼻病毒、呼吸道合胞病毒（RSV）、流感病毒、副流感病毒、冠状病毒和腺病毒在儿童急性呼吸道感染中的频率。
本研究纳入了 109 例患有呼吸道感染的儿科患者。实时 PCR、DFA 和细胞培养法分别用于检测肺炎衣原体、RSV 抗原和流感病毒。多重 PCR 用于检测其他病毒。
在样本中未检测到肺炎衣原体 DNA。在 109 例喉拭子中检测到病毒 43 例（43/109，39.4%）。检测到的病毒种类的频率顺序如下：鼻病毒 14.7%、RSV B 7.3%、流感 A 6.4%、副流感病毒 3.6%、腺病毒 3.6%、冠状病毒 0.9%、副流感病毒 3 型 0.9%、副流感病毒 4 型 0.9%和 RSV A 0.9%。PCR 和 DFA 方法诊断 RSV 感染的敏感性分别为 100%和 100%。PCR 和 DFA 方法诊断 RSV 感染的特异性分别为 97%和 100%。PCR 和细胞培养法检测流感病毒感染的敏感性均为 100%，特异性分别为 96%和 100%。
病毒病原体的检出率为 39.4%。流感病毒和 RSV 较为常见。副流感病毒也很常见（3.6%）。肺炎衣原体不是儿童急性呼吸道疾病的常见病原体。
