Department of Pediatrics and Adolescence Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Pediatr Res. 2012 Jan;71(1):46-53. doi: 10.1038/pr.2011.11.
To examine the immune-modulatory effects of probiotics during early infancy, Bifidobacterium breve M-16V (B. breve) was administered to rat pups during the newborn or weaning period, and the expression of inflammatory genes was investigated using a cDNA microarray and real-time PCR.
After B. breve administration, significant increases in the numbers of Bifidobacterium in both the cecum and colon were confirmed during the newborn period. The numbers of upregulated and downregulated genes were greater during the weaning period than in the newborn period and were greatest in the colon, with fewer genes altered in the small intestine and the fewest in the spleen. The expression of inflammation-related genes, including lipoprotein lipase (Lpl), glutathione peroxidase 2 (Gpx2), and lipopolysaccharide-binding protein (Lbp), was significantly reduced in the colon during the newborn period. In weaning rat pups, the expression of CD3d, a cell surface receptor-linked signaling molecule, was significantly enhanced in the colon; however, the expression of co-stimulatory molecules was not enhanced.
Our findings support a possible role for B. breve in mediating anti-inflammatory and antiallergic reactions by modulating the expression of inflammatory molecules during the newborn period and by regulating the expression of co-stimulatory molecules during the weaning period.
Gene expression in the intestine was investigated after feeding 5 × 10(8) cfu of B. breve every day to the F344/Du rat from days 1 to 14 (newborn group) and from days 21 to 34 (weaning group). mRNA was extracted from intestine, and the expression of inflammatory gene was analyzed by microarray and real-time PCR.
为了研究益生菌在婴儿早期的免疫调节作用，在新生期或断奶期给新生大鼠灌胃短双歧杆菌 M-16V（B. breve），并用 cDNA 微阵列和实时 PCR 研究炎症基因的表达。
B. breve 给药后，新生期盲肠和结肠中双歧杆菌数量明显增加。与新生期相比，断奶期上调和下调的基因数量更多，且在结肠中最多，在小肠中改变的基因较少，在脾脏中最少。炎症相关基因的表达，包括脂蛋白脂肪酶（Lpl）、谷胱甘肽过氧化物酶 2（Gpx2）和脂多糖结合蛋白（Lbp），在新生期的结肠中显著降低。在断奶大鼠中，CD3d，一种细胞表面受体相关信号分子，在结肠中的表达显著增强；然而，共刺激分子的表达没有增强。
我们的研究结果支持 B. breve 通过调节新生期炎症分子的表达和调节断奶期共刺激分子的表达，在介导抗炎和抗过敏反应中发挥作用。
每天给 F344/Du 大鼠灌胃 5×10(8)cfu 的 B. breve，从第 1 天到第 14 天（新生组）和第 21 天到第 34 天（断奶组），从肠道中提取 mRNA，并用微阵列和实时 PCR 分析炎症基因的表达。
