Bedoya Astrid M, Jaramillo Roberto, Baena Armando, Castaño Jorge, Olaya Natalia, Zea Arnold H, Herrero Rolando, Sanchez Gloria I
Grupo Infección y Cáncer, Facultad de Medicina, Universidad de Antioquia, Cra 51D No. 62-29 Lab 283, Medellin, Colombia.
Escuela de Microbiología, Universidad de Antioquia, Medellin, Colombia.
Cancer Microenviron. 2013 Apr;6(1):69-77. doi: 10.1007/s12307-012-0097-8. Epub 2012 Jan 31.
Only a small proportion of women infected with Human Papillomavirus (HPV) develop cervical cancer. Host immune response seems to play a role eliminating the viral infection and preventing progression to cancer. Characterization of tumor infiltrating lymphocytes (TILs) in cervical pre-neoplastic lesions and cervical cancer may be helpful to understand the mechanisms that mediate this protection. The aim of this study was to determine if there are differences in the localization and density (cells/mm(2)) of CD8+ T-cells, CD4+ T-cells and Tregs (CD25 + Foxp3+) in cervical pre-neoplastic lesions and cervical cancer. Immunohistochemical analysis of sections of 96 (26 CIN1, 21 CIN2, 25 CIN3, and 24 SCC) samples revealed that regardless of CIN grades, CD8+ T-cells are more abundant than CD4+, CD25+ and Foxp3+ cells in both the stroma and epithelium. There was a higher density of CD8+ cells in the stroma of cervical cancer compared to CIN3 (OR = 4.20, 95% CI 1.2-15), CIN2 (OR = 7.86, 95% CI 1.7-36.4) and CIN1 (OR = 4.25, 95% CI 1.1-17). Studies evaluating whether these cells are recruited before or after cancer progression will be helpful to understand the role of these cells in the natural history of HPV-induced lesions.
只有一小部分感染人乳头瘤病毒（HPV）的女性会患上宫颈癌。宿主免疫反应似乎在消除病毒感染和预防癌症进展方面发挥作用。对宫颈癌前病变和宫颈癌中的肿瘤浸润淋巴细胞（TILs）进行特征分析，可能有助于了解介导这种保护作用的机制。本研究的目的是确定宫颈前病变和宫颈癌中CD8 + T细胞、CD4 + T细胞和调节性T细胞（CD25 + Foxp3 +）在定位和密度（细胞/mm²）上是否存在差异。对96个样本（26个CIN1、21个CIN2、25个CIN3和24个鳞状细胞癌）的切片进行免疫组织化学分析显示，无论CIN分级如何，基质和上皮中的CD8 + T细胞均比CD4 +、CD25 +和Foxp3 +细胞丰富。与CIN3（OR = 4.20，95% CI 1.2 - 15）、CIN2（OR = 7.86，95% CI 1.7 - 36.4）和CIN1（OR = 4.25，95% CI 1.1 - 17）相比，宫颈癌基质中CD8 +细胞的密度更高。评估这些细胞是在癌症进展之前还是之后被招募的研究，将有助于了解这些细胞在HPV诱导病变自然史中的作用。
