Kraus R J, Shinnick F L, Marlett J A
Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
J Chromatogr. 1990 Jul 27;513:71-81. doi: 10.1016/s0021-9673(01)89426-7.
A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO3, reduced with sodium borohydride, acidified, evaporated and alditol acetates prepared. Baseline resolution was achieved on a glass-capillary SP-2340 column in ca. 52 min. Reproducibility, response factors and hydrolysis losses were determined. Quantitation was linear over the range of 10-20 micrograms/ml to 2000 micrograms/ml. Conditions were defined for the reproducible quantitation of muramic acid.
