Neeser J R, Schweizer T F
Anal Biochem. 1984 Oct;142(1):58-67. doi: 10.1016/0003-2697(84)90516-5.
Complete gas-liquid chromatographic separation of O-methyloxime acetates (syn and anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biological material (less than or equal to 2.5 X 10(-3) M sugar solution in 4 N trifluoroacetic acid at 125 degrees C for 1 h) has been developed, and results have been compared with those obtained with standard hydrolytic conditions in order to ensure complete release of amino sugars from glycoproteins, together with minimum losses of neutral sugars. The combination of this acid hydrolysis with the above improved derivatization procedure for the gas-liquid chromatographic analysis has led to a simple, rapid, and sensitive analytical method, which has been successfully tested on three glycoproteins (fetuin, mucin, and peroxidase) and two plant cell-wall polysaccharide fractions (soluble fibers from carrots and soybeans).
使用熔融石英聚乙二醇20M毛细管柱，已实现了由八种中性糖、三种己糖胺和胞壁酸制备的O-甲基肟乙酸酯（顺式和反式异构体）的完全气-液色谱分离。已开发出一种针对含碳水化合物生物材料的单一水解步骤（在4N三氟乙酸中，糖溶液浓度小于或等于2.5×10⁻³M，于125℃水解1小时），并将结果与标准水解条件下获得的结果进行比较，以确保从糖蛋白中完全释放氨基糖，同时使中性糖的损失降至最低。这种酸水解与上述改进的气-液色谱分析衍生化程序相结合，产生了一种简单、快速且灵敏的分析方法，该方法已成功应用于三种糖蛋白（胎球蛋白、粘蛋白和过氧化物酶）以及两种植物细胞壁多糖组分（胡萝卜和大豆的可溶性纤维）的检测。
