Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Methods. 2012 Jul;57(3):318-30. doi: 10.1016/j.ymeth.2012.01.003. Epub 2012 Jan 24.
Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.
荧光蛋白现在是生物医学研究各个领域的关键工具。在本文中，我们回顾了使用荧光蛋白进行流式细胞术所需的技术，特别专注于每种蛋白的激发和发射要求，以及为了实现最佳使用效果所需的特定设备。
