Central Laboratory, China Medical University, Heping District, Shenyang, PR China.
Int J Biol Macromol. 2011 Nov 1;49(4):693-9. doi: 10.1016/j.ijbiomac.2011.06.029. Epub 2011 Jul 7.
To investigate and analyze induction of phenotypic and functional maturation of murine DCs by GLP. Both phenotypic and functional activities were assessed with use of conventional scanning electronic microscopy (SEM) for the morphology of the DCs, transmitted electron microscopy (TEM) for intracellular lysosomes inside the DC, cellular immunohistochemistry for phagocytosis by the DCs, flow cytometry (FCM) for the changes in key surface molecules, bio-assay for the activity of acid phosphatases (ACP), and ELISA for the production of pro-inflammatory cytokine IL-12. It was found that GLP induced phenotypic maturation, as evidenced by increased expression of CD86, CD40, and MHC II. Functional experiments showed the down-regulation of ACP inside the DCs, which occurs when phagocytosis of DCs decreased, and antigen presentation increased with maturation. Finally, GLP increased the production of IL-12. These data reveals that GLP promotes effective activation of murine DCs. This adjuvant-like activity may have therapeutic applications in clinical settings that require a boosting of the immune response. Therefore concluded that GLP can exert positive induction to murine DCs at the used concentration.
为了研究和分析 GLP 对鼠源性树突状细胞（DC）表型和功能成熟的诱导作用。通过常规扫描电子显微镜（SEM）评估 DC 形态、透射电子显微镜（TEM）评估 DC 内的细胞内溶酶体、细胞免疫组化评估 DC 的吞噬作用、流式细胞术（FCM）评估关键表面分子的变化、生物测定法评估酸性磷酸酶（ACP）的活性、以及 ELISA 法评估促炎细胞因子 IL-12 的产生。结果发现，GLP 诱导了表型成熟，表现为 CD86、CD40 和 MHC II 的表达增加。功能实验表明，当 DC 的吞噬作用减少而抗原呈递增加时，DC 内的 ACP 下调。最后，GLP 增加了 IL-12 的产生。这些数据表明，GLP 可促进鼠源性 DC 的有效激活。这种佐剂样活性在需要增强免疫反应的临床环境中可能具有治疗应用。因此得出结论，GLP 可以在使用浓度下对鼠源性 DC 发挥积极诱导作用。
