Vitković L, Kalebic T, de Cunha A, Fauci A S
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
J Neuroimmunol. 1990 Dec;30(2-3):153-60. doi: 10.1016/0165-5728(90)90099-9.
Human promonocytic cells chronically infected with human immunodeficiency virus-1 (HIV-1) (clone U1.1.5) were grown in the presence of media conditioned by primary rat cortical astrocytes and HIV-1 expression was assessed by measuring reverse transcriptase activity. Media conditioned by non-stimulated and lipopolysaccharide (LPS)-stimulated astrocytes induced the expression of HIV-1 2.1-fold and 4.1-fold, respectively. LPS alone, media conditioned by the uninfected parental cell line of U1.1.5 (U937), and culture media from four other cell lines, had no effect on viral expression. The magnitude of induction was time- and dose-dependent. Tumor necrosis factor alpha (TNF-alpha) was detected in LPS-stimulated astrocyte-conditioned medium and the HIV-inducing capability of the medium was neutralized, in part, by an antibody to recombinant murine TNF-alpha. These results suggest a role for astrocytes in the induction of HIV expression and thus in the pathogenesis of HIV-1 infection in brain.
用人免疫缺陷病毒1型（HIV-1）（克隆U1.1.5）慢性感染的人原单核细胞，在经原代大鼠皮质星形胶质细胞预处理的培养基中培养，并通过测量逆转录酶活性评估HIV-1的表达。未经刺激和经脂多糖（LPS）刺激的星形胶质细胞预处理的培养基，分别使HIV-1表达诱导了2.1倍和4.1倍。单独的LPS、U1.1.5未感染亲本细胞系（U937）预处理的培养基以及其他四种细胞系的培养基，对病毒表达均无影响。诱导程度呈时间和剂量依赖性。在LPS刺激的星形胶质细胞预处理培养基中检测到肿瘤坏死因子α（TNF-α），并且该培养基的HIV诱导能力部分被抗重组鼠TNF-α抗体中和。这些结果表明星形胶质细胞在HIV表达诱导中起作用，因而在脑内HIV-1感染的发病机制中起作用。
