Vitković L, Wood G P, Major E O, Fauci A S
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
AIDS Res Hum Retroviruses. 1991 Sep;7(9):723-7. doi: 10.1089/aid.1991.7.723.
Human promonocyte cells chronically infected with human immunodeficiency virus type (HIV-1) (clone U1.1.5) were grown in the presence of media conditioned by human astrocytes and glioma cell lines U251 and 253. HIV-1 expression was assessed by measuring reverse transcriptase activity. All media conditioned by unstimulated and lipopolysaccharide (LPS) stimulated glial cells induced HIV-1 expression and contained detectable levels of interleukin-6 (IL-6) but not tumor necrosis factor-alpha (TNF-alpha). An antibody against IL-6, but not against TNF-alpha, reduced the induction of HIV-1 by the conditioned media in a concentration-dependent manner. The magnitude of HIV-1 induction by the conditioned media was proportional to the concentration of IL-6 in them. The data indicate that normal and transformed human astrocytes are capable of stimulating HIV-1 expression in chronically infected promonocytic cells by secreting IL-6. The results demonstrate that cytokines secreted by neural cells could play an important role in regulating HIV-1 expression in the brain.
用人星形胶质细胞和胶质瘤细胞系U251及253处理过的培养基培养长期感染1型人类免疫缺陷病毒（HIV-1）（克隆U1.1.5）的人原单核细胞。通过测量逆转录酶活性评估HIV-1表达。所有未经刺激和经脂多糖（LPS）刺激的神经胶质细胞处理过的培养基均诱导HIV-1表达，并含有可检测水平的白细胞介素-6（IL-6），但不含有肿瘤坏死因子-α（TNF-α）。抗IL-6抗体而非抗TNF-α抗体以浓度依赖方式降低了处理过的培养基对HIV-1的诱导。处理过的培养基对HIV-1的诱导程度与其中IL-6的浓度成正比。数据表明，正常和转化的人星形胶质细胞能够通过分泌IL-6刺激长期感染的原单核细胞中的HIV-1表达。结果表明，神经细胞分泌的细胞因子可能在调节脑中HIV-1表达方面发挥重要作用。
