Nutrition, Clinic, Institute of Health Sciences, Superior Teaching Establishment Federation University (Universidade FEEVALE), Novo Hamburgo, Rio Grande do Sul, Brazil.
J Physiol Biochem. 2012 Sep;68(3):365-75. doi: 10.1007/s13105-012-0148-0.
Heat shock protein 70 (HSP70) is a chaperone that maintains protein conformation during heat stress. It has recently been observed that HSP70 may be released from cells in response to increased energy demand (e.g., exercise) and/or oxidative stress. Since HSP70 levels should change in response to athletic training, we have investigated whether blood HSP70 levels in young women handball players change over a complete training season. Thirty women handball players (12-24 years old) were divided into low (≥30 pg mL(-1)) (LE) and normal (30-330 pg mL(-1)) (NE) estradiol groups. HSP70 levels in lymphocytes and plasma and blood redox parameters were evaluated over 1 year (2009), with sampling at the beginning, middle, and end of the season. We observed no changes in superoxide dismutase activity or protein carbonyl or extracellular HSP70 levels, while catalase activity increased at the middle of the season in the NE group, and the thiobarbituric acid species levels in both groups were higher at the beginning of the season than at the middle or end. The lymphocyte HSP70 content was higher at the middle and end than at the beginning of the season in the NE group and also higher in the LE group than in the NE group at the beginning of the season. These results suggest that plasma estradiol levels may play an important role in exercise training and that the intracellular HSP70 content, a biomarker for inflammation, is affected by both estradiol levels and exercise-induced oxidative stress.
热休克蛋白 70（HSP70）是一种伴侣蛋白，可在热应激时维持蛋白质构象。最近观察到，HSP70 可能会响应增加的能量需求（例如运动）和/或氧化应激而从细胞中释放。由于 HSP70 水平应随运动训练而变化，我们研究了年轻女子手球运动员的血液 HSP70 水平是否在整个训练季节中发生变化。将 30 名女子手球运动员（12-24 岁）分为低（≥30 pg mL（-1））（LE）和正常（30-330 pg mL（-1））（NE）雌二醇组。在 1 年（2009 年）中评估了淋巴细胞和血浆中的 HSP70 水平以及血液氧化还原参数，在赛季开始、中间和结束时进行采样。我们没有观察到超氧化物歧化酶活性或蛋白质羰基或细胞外 HSP70 水平的变化，而 NE 组在赛季中期的过氧化氢酶活性增加，并且两个组的硫代巴比妥酸物质水平在赛季开始时均高于中间或结束时。NE 组的淋巴细胞 HSP70 含量在赛季中间和结束时高于开始时，并且在 LE 组中也高于 NE 组在开始时的水平。这些结果表明，血浆雌二醇水平可能在运动训练中起重要作用，并且细胞内 HSP70 含量（炎症的生物标志物）受雌二醇水平和运动引起的氧化应激的影响。
