Li Ping, Xu Dandan, Peng Zuogang, Zhang Yaoguang
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Beibei, Chongqing 400715, China.
Mitochondrial DNA. 2012 Feb;23(1):34-6. doi: 10.3109/19401736.2011.643880.
The complete mitogenome sequence of the spotted steed (Hemibarbus maculatus) was determined using long PCR reactions. The genome is 16,611 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of H. maculatus was similar to that of most other vertebrates. The base composition of H-strand in descending order is A (29.16%), C (27.41%), T (25.93%), and G (17.50%), with an AT content of 55.09%. The Kimura two-parameter distances between mitogenome sequences of H. maculatus and Hemibarbus labeo, Hemibarbus barbus, Hemibarbus longirostris, Hemibarbus mylodon were 2.09%, 2.35%, 12.77%, and 13.92%, respectively. The phylogenetic relationships among the five Hemibarbus species based on the whole mitogenome sequence data were (((H. barbus, H. labeo), H. maculatus), (H. longirostris, H. mylodon)).
利用长链PCR反应测定了斑鳠（Hemibarbus maculatus）的完整线粒体基因组序列。该基因组长度为16,611 bp，由13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区组成。斑鳠的基因顺序和组成与大多数其他脊椎动物相似。H链的碱基组成按降序排列为A（29.16%）、C（27.41%）、T（25.93%）和G（17.50%），AT含量为55.09%。斑鳠与唇鱼骨（Hemibarbus labeo）、棒花鱼（Hemibarbus barbus）、长吻鳠（Hemibarbus longirostris）、细鳞斜颌鲴（Hemibarbus mylodon）线粒体基因组序列之间的Kimura双参数距离分别为2.09%、2.35%、12.77%和13.92%。基于完整线粒体基因组序列数据的五种鳠属鱼类之间的系统发育关系为（（（棒花鱼，唇鱼骨），斑鳠），（长吻鳠，细鳞斜颌鲴））。
