利用下一代测序方法鉴定丙型肝炎病毒传播。
Identification of hepatitis C virus transmission using a next-generation sequencing approach.
机构信息
Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico City, Mexico.
出版信息
J Clin Microbiol. 2012 Apr;50(4):1461-3. doi: 10.1128/JCM.00005-12. Epub 2012 Feb 1.
Abstract
Here, we describe a transmission event of hepatitis C virus (HCV) among injection drug users. Next-generation sequencing (NGS) was used to assess the intrahost viral genetic variation. Deep amplicon sequencing of HCV hypervariable region 1 allowed for a detailed analysis of the structure of the viral population. Establishment of the genetic relatedness between cases was accomplished by phylogenetic analysis. NGS is a powerful tool with applications in molecular epidemiology studies and outbreak investigations.
摘要
在这里，我们描述了一起丙型肝炎病毒（HCV）在注射吸毒者中的传播事件。我们使用下一代测序（NGS）来评估病毒宿主内的遗传变异。对 HCV 高变区 1 的深度扩增子测序允许对病毒群体的结构进行详细分析。通过系统进化分析确定病例之间的遗传关系。NGS 是一种强大的工具，可应用于分子流行病学研究和疫情调查。
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