Veterans Affairs Palo Alto Healthcare System, California 94304, USA.
Genes Dev. 2012 Feb 15;26(4):338-43. doi: 10.1101/gad.182121.111. Epub 2012 Feb 2.
Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti-differentiation ncRNA) as an 855-base-pair lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis.
长非编码 RNA（lncRNAs）调节多种过程，但 lncRNAs 在维持体组织祖细胞未分化状态中的潜在作用仍未被描述。我们使用转录组测序和平铺阵列比较了表皮祖细胞群体与分化细胞中的 lncRNA 表达。我们将 ANCR（抗分化 ncRNA）鉴定为在分化过程中下调的 855 个碱基对的 lncRNA。在不施加任何其他刺激的情况下，在含有祖细胞的群体中耗尽 ANCR 会导致快速诱导分化基因。在表皮中，ANCR 的缺失消除了正常情况下从含有祖细胞的隔室中排除分化的现象。因此，ANCR lncRNA 是维持表皮中未分化细胞状态所必需的。
