Rempel Lea A, Freking Brad A, Miles Jeremy R, Nonneman Dan J, Rohrer Gary A, Schneider James F, Vallet Jeffrey L
U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture Clay Center, NE, USA.
Front Genet. 2011 May 4;2:20. doi: 10.3389/fgene.2011.00020. eCollection 2011.
The ovary and placenta are dynamic structures requiring constant modification both structurally and through cell-cell communication capabilities. The extracellular matrix and basement membranes are primarily composed of a milieu of glycosaminoglycans, including heparan sulfate and hyaluronan. Heparanase (HPSE) and hyaluronidases (HYAL) are responsible for degrading heparan sulfate and hyaluronan, respectively. Therefore, the objective of this study was to evaluate the relationship of SNPs distinct to HPSE, HYAL1, and HYAL2 with measurements of reproduction and production traits in swine. Single trait associations were performed on a Landrace-Duroc-Yorkshire population using SNPs discovered and identified in HPSE, HYAL1, and HYAL2. Analyses were conducted on an extended pedigree and SNPs were found to be associated with reproductive and production traits. Prior to multiple-testing corrections, SNPs within HPSE were weakly associated (P < 0.03) having additive effects with age at puberty (-2.5 ± 1.08 days), ovulation rate (0.5 ± 0.24 corpora lutea), and number of piglets born alive (0.9 ± 0.44 piglets). A HYAL1 and two HYAL2 SNP were nominally associated (P ≤ 0.0063) with number of piglets born alive after multiple-testing corrections (effects between 1.02 and 1.44 piglets), while one of the same HYAL2 markers maintained a modest association (P = 0.0043) having a dominant effect with number of piglets weaned (1.2 ± 0.41 piglets) after multiple-testing correction. Functionally, HPSE and HYAL1 and 2 have been shown to participate in events related to ovarian and placental activity. SNPs from these studies could potentially assist with understanding genetic components underlying sow lifetime productivity as measured by piglet survivability based on number born alive and number weaned, thereby contributing to a greater number of pigs/sow/year.
卵巢和胎盘是动态结构，在结构上以及通过细胞间通讯能力都需要不断进行调整。细胞外基质和基底膜主要由包括硫酸乙酰肝素和透明质酸在内的糖胺聚糖环境组成。乙酰肝素酶（HPSE）和透明质酸酶（HYAL）分别负责降解硫酸乙酰肝素和透明质酸。因此，本研究的目的是评估HPSE、HYAL1和HYAL2特有的单核苷酸多态性（SNP）与猪繁殖和生产性状测定之间的关系。使用在HPSE、HYAL1和HYAL2中发现并鉴定的SNP，对长白-杜洛克-约克夏猪群体进行单性状关联分析。在一个扩展的系谱上进行分析，发现SNP与繁殖和生产性状相关。在进行多重检验校正之前，HPSE内的SNP与初情期年龄（-2.5±1.08天）、排卵率（0.5±0.24个黄体）和活产仔猪数（0.9±0.44头仔猪）呈弱关联（P＜0.03），具有加性效应。经过多重检验校正后，一个HYAL1和两个HYAL2 SNP与活产仔猪数呈名义上的关联（P≤0.0063）（效应在1.02至1.44头仔猪之间），而相同的HYAL2标记之一在经过多重检验校正后与断奶仔猪数呈适度关联（P = 0.0043），具有显性效应（1.2±0.41头仔猪）。在功能上，HPSE以及HYAL1和HYAL2已被证明参与与卵巢和胎盘活动相关的事件。这些研究中的SNP可能有助于理解基于活产数和断奶数的仔猪存活率所衡量的母猪终生生产力的遗传成分，从而有助于提高每年每头母猪的产仔数。
