Key Laboratory of Aquatic Biotechnology, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.
PLoS One. 2012;7(1):e30925. doi: 10.1371/journal.pone.0030925. Epub 2012 Jan 27.
The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play important roles in vertebrate development. They regulate gene expression through sequence-specific interactions with the 3' untranslated regions (UTRs) of target mRNAs and thereby cause translational repression or mRNA destabilization. Intriguingly, the role of miRNAs in the skeletal muscle development of the common carp remains unknown. In this study, a small-RNA cDNA library was constructed from the skeletal muscle of the common carp, and Solexa sequencing technology was used to perform high throughput sequencing of the library. Subsequent bioinformatics analysis identified 188 conserved miRNAs and 7 novel miRNAs in the carp skeletal muscle. The miRNA expression profiling showed that, miR-1, miR-133a-3p, and miR-206 were specifically expressed in muscle-containing organs, and that miR-1, miR-21, miR-26a, miR-27a, miR-133a-3p, miR-206, miR-214 and miR-222 were differentially expressed in the process of skeletal muscle development of the common carp. This study provides a first identification and profiling of miRNAs related to the muscle biology of the common carp. Their identification could provide clues leading towards a better understanding of the molecular mechanisms of carp skeletal muscle development.
鲤鱼是世界淡水养殖业中最重要的养殖品种之一。由于其骨骼肌质量高，该物种的养殖特别高产；然而，鲤鱼骨骼肌发育的分子机制尚不清楚。已经表明，一类称为 microRNAs（miRNAs）的非编码约 22 个核苷酸的 RNA 在脊椎动物发育中发挥重要作用。它们通过与靶 mRNAs 的 3'非翻译区（UTR）的序列特异性相互作用来调节基因表达，从而导致翻译抑制或 mRNA 不稳定。有趣的是，miRNAs 在鲤鱼骨骼肌发育中的作用尚不清楚。在这项研究中，从鲤鱼的骨骼肌中构建了一个 small-RNA cDNA 文库，并使用 Solexa 测序技术对文库进行高通量测序。随后的生物信息学分析在鲤鱼骨骼肌中鉴定了 188 个保守的 miRNAs 和 7 个新的 miRNAs。miRNA 表达谱分析表明，miR-1、miR-133a-3p 和 miR-206 特异性表达在含有肌肉的器官中，而 miR-1、miR-21、miR-26a、miR-27a、miR-133a-3p、miR-206、miR-214 和 miR-222 在鲤鱼骨骼肌发育过程中差异表达。本研究首次鉴定和分析了与鲤鱼肌肉生物学相关的 miRNAs。它们的鉴定可以为更好地理解鲤鱼骨骼肌发育的分子机制提供线索。
