Fonseca Priscilla Duarte Marques, Corral Marcelo Andreeta, Meisel Dirce Mary C Lima, Levi Debora, Nascimento Rafael Correa, Castro-Borges William, Gryschek Ronaldo, Costa-Cruz Julia Maria, Paula Fabiana Martins de
Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2020;62:e23. doi: 10.1590/s1678-9946202062023. Epub 2020 May 11.
In experimental infection with Strongyloides venezuelensis, the acute and recovery phases can be distinguished, unlike human infections caused by Strongyloides stercoralis. The objective of this study was to evaluate the production of anti-Strongyloides IgG antibodies and the recognition of immunogenic protein bands during the acute and the recovery phases in rats experimentally infected with S. venezuelensis. Rats were infected subcutaneously with 400 or 4,000 S. venezuelensis infective larvae. The acute phase was characterized by elimination of a large number of eggs in the faeces on days 6-14 post infection; the recovery phase was characterized by the resolution of the infection between days 30 and 35 post infection. Differences in IgG levels were observed in the acute and the recovery phases. Different antigenic fractions were recognized in both phases of infection. It is concluded that proteins within the 30-40 kDa range are immunoreactive markers for both the acute and the recovery phases in rats experimentally infected with S. venezuelensis, particularly using membrane antigen.
在委内瑞拉类圆线虫的实验性感染中，可以区分急性期和恢复期，这与粪类圆线虫引起的人类感染不同。本研究的目的是评估实验感染委内瑞拉类圆线虫的大鼠在急性期和恢复期抗类圆线虫IgG抗体的产生以及免疫原性蛋白条带的识别情况。大鼠皮下接种400或4000条委内瑞拉类圆线虫感染性幼虫。急性期的特征是感染后第6 - 14天粪便中排出大量虫卵；恢复期的特征是感染后第30 - 35天感染得到解决。在急性期和恢复期观察到IgG水平存在差异。在感染的两个阶段都识别出了不同的抗原组分。得出的结论是，在实验感染委内瑞拉类圆线虫的大鼠中，30 - 40 kDa范围内的蛋白质是急性期和恢复期的免疫反应性标志物，特别是使用膜抗原时。
