烟曲霉过敏原I是一种主要的IgE结合蛋白，属于细胞毒素米托吉林家族的成员。
Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins.
作者信息
Arruda L K, Platts-Mills T A, Fox J W, Chapman M D
机构信息
Department of Internal Medicine, University of Virginia, Charlottesville 22908.
出版信息
J Exp Med. 1990 Nov 1;172(5):1529-32. doi: 10.1084/jem.172.5.1529.
Abstract
A major 18-kD IgE-binding protein from Aspergillus fumigatus (Asp fI) has been purified. Partial amino acid sequencing of Asp f I showed extensive sequence homology (95%) between Asp fI and a cytotoxin (mitogillin) produced by A. restrictus. Crossinhibition radioimmunoassay using murine monoclonal antibody and human IgG and IgE antibodies showed that Asp fI and mitogillin were antigenically indistinguishable. Furthermore, both proteins inhibited protein synthesis in vitro by greater than 90%. Asp fI was expressed in A. fumigatus but not in seven other Aspergillus species. The results suggest that Asp fI could play a dual role in the pathogenesis of A. fumigatus-related diseases by promoting colonization through cytotoxic activity and by causing inflammatory reactions involving IgE antibodies.
摘要
来自烟曲霉的一种主要的18-kD IgE结合蛋白（Asp fI）已被纯化。Asp fI的部分氨基酸序列分析表明，Asp fI与局限曲霉产生的一种细胞毒素（丝裂毒素）之间存在广泛的序列同源性（95%）。使用鼠单克隆抗体以及人IgG和IgE抗体进行的交叉抑制放射免疫测定表明，Asp fI和丝裂毒素在抗原性上无法区分。此外，这两种蛋白在体外均能抑制超过90%的蛋白质合成。Asp fI在烟曲霉中表达，但在其他七种曲霉属物种中不表达。结果表明，Asp fI可能在烟曲霉相关疾病的发病机制中发挥双重作用，即通过细胞毒性活性促进定植，并通过引发涉及IgE抗体的炎症反应。
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本文引用的文献
1
Antibodies against Aspergillus fumigatus II. Identification and quantification by means of crossed immunoelectrophoresis.
Int Arch Allergy Appl Immunol. 1980;62(3):265-75.
2
Purification and characterization of the major allergen from Dermatophagoides pteronyssinus-antigen P1.来自粉尘螨的主要变应原——抗原P1的纯化与特性分析
J Immunol. 1980 Aug;125(2):587-92.
3
Immunosuppression in vitro by a metabolite of a human pathogenic fungus.一种人类致病真菌的代谢产物在体外的免疫抑制作用。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3835-7. doi: 10.1073/pnas.81.12.3835.
4
Evaluation of mitogillin (NSC-69529) in the treatment of naturally occurring canine neoplasms.
Cancer Chemother Rep. 1971 Apr;55(2):101-13.
5
The mode of action of restrictocin and mitogillin on eukaryotic ribosomes. Inhibition of brain protein synthesis, cleavage and sequence of the ribosomal RNA fragment.限制酶和嗜蓝孢孔菌毒素对真核核糖体的作用方式。对脑蛋白合成的抑制、核糖体RNA片段的切割及序列分析。
Eur J Biochem. 1985 May 15;149(1):29-34. doi: 10.1111/j.1432-1033.1985.tb08888.x.
6
Complete amino acid sequence of the Aspergillus cytotoxin mitogillin.曲霉菌细胞毒素米托吉林的完整氨基酸序列。
Biochemistry. 1985 Feb 12;24(4):861-7. doi: 10.1021/bi00325a008.
7
A pathologic study of allergic bronchopulmonary aspergillosis.
J Allergy Clin Immunol. 1988 Apr;81(4):718-25. doi: 10.1016/0091-6749(88)91044-5.
8
Epidemiology of acute asthma: IgE antibodies to common inhalant allergens as a risk factor for emergency room visits.急性哮喘的流行病学：针对常见吸入性过敏原的IgE抗体作为急诊就诊的危险因素。
J Allergy Clin Immunol. 1989 May;83(5):875-82. doi: 10.1016/0091-6749(89)90100-0.
9
Antibody response to low-molecular-weight antigens of Aspergillus fumigatus in allergic bronchopulmonary aspergillosis.变应性支气管肺曲霉病中针对烟曲霉低分子量抗原的抗体反应
J Clin Microbiol. 1989 Jun;27(6):1312-6. doi: 10.1128/jcm.27.6.1312-1316.1989.
10
Aspergillus, asthma, and amoebae.
Lancet. 1989 Apr 22;1(8643):893-4. doi: 10.1016/s0140-6736(89)92877-8.
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