快速简单的厌氧氨氧化菌的冷冻保存。
Rapid and simple cryopreservation of anaerobic ammonium-oxidizing bacteria.
机构信息
Ghent University, Department of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent, Belgium.
出版信息
Appl Environ Microbiol. 2012 Apr;78(8):3010-3. doi: 10.1128/AEM.07501-11. Epub 2012 Feb 3.
Abstract
A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of "Candidatus Kuenenia stuttgartiensis."
摘要
本研究建立了一种快速简单的厌氧氨氧化菌（Anammox 菌）长期低温保存方案。经过 29 周的-80°C 保存后，所有测试培养物在至少一组应用保存条件下都观察到了活性恢复。“Kuenenia stuttgartiensis”的单细胞培养也证明了生长恢复。
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