Russian Cardiology Research Complex, 3rd Cherepkovskaya street, 15a, Moscow, Russia 121552.
Mol Cell Biochem. 2012 Jun;365(1-2):93-8. doi: 10.1007/s11010-012-1247-5. Epub 2012 Feb 4.
In this study, we show that low density lipoproteins (LDL) from human blood plasma which was oxidized by animal C-15 lipoxygenase is taken up by cultivated human macrophages with the same effectiveness as with non-oxidized (native) LDL. At the same time malonyldialdehyde-modified LDL is captured by cultivated macrophages very actively. Based on differences in catabolism of LDL with various levels of primary and secondary products of free-radical oxidation, it was offered to discriminate between the oxidized LDL itself (lipohydroperoxide-rich LDL) and the LDL that was chemically modified by free-radical oxidation secondary products of aldehyde nature. In this respect, aldehyde-modified but not oxidized (lipohydroperoxide-containing) LDL is atherogenic.
在这项研究中，我们表明，用动物 C-15 脂氧合酶氧化后的人血浆低密度脂蛋白（LDL）与未经氧化的（天然）LDL 一样被培养的人巨噬细胞摄取。同时，丙二醛修饰的 LDL 被培养的巨噬细胞非常有效地捕获。基于 LDL 不同程度的一级和二级自由基氧化产物的代谢差异，提出区分氧化 LDL 本身（富含脂氢过氧化物的 LDL）和自由基氧化次级产物化学修饰的 LDL。在这方面，醛修饰但不氧化（含有脂氢过氧化物）的 LDL 具有致动脉粥样硬化作用。
