Entomology Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
J Med Entomol. 2012 Jan;49(1):107-11. doi: 10.1603/me11092.
The toxicity of pellitorine alone or in combination with (-)-asarinin, alpha-asarone, methyleugenol, or pentadecane (1:1, 1:2, 1:3, 2:1, and 3:1 ratios) to third instars from an insecticide-susceptible KS-CP strain and -resistant DJ-CP colony of Culex pipiens pallens Coquillett was evaluated using a direct-contact mortality bioassay. The binary mixture of pellitorine and (-)-asarinin (3:1 ratio) was significantly more toxic against KS-CP larvae (0.95 mg/liter) and DJ-CP larvae (1.07 mg/liter) than either pellitorine (2.08 mg/liter for KS-CP and 2.33 mg/liter for DJ-CP) or (-)-asarinin (11.45 and 12.61 mg/liter) alone. The toxicity of the other binary mixtures (1:1, 1:2, 1:3, and 2:1 ratios) and pellitorine did not differ significantly from each other. Based on the co-toxicity coefficient (CC) and synergistic factor (SF), the three binary mixtures (1:3, 2:1, and 3:1) operated synergistically (CC, 250-390 and SF, 1.4-2.2 for KS-CP; CC, 257-279 and SF, 1.1-2.1 for DJ-CP). The binary mixtures of pellitorine and (-)-asarinin merit further study as potential larvicides for the control of insecticide-resistant mosquito populations.
采用直接接触致死生物测定法，评价了花椒毒素单独以及与（-）-细辛脂素、α-细辛脑、甲基丁香酚或十五烷（1:1、1:2、1:3、2:1 和 3:1 比例）组合对来自杀虫剂敏感的 KS-CP 品系和抗性 DJ-CP 品系库蚊三龄幼虫的毒性。花椒毒素和（-）-细辛脂素（3:1 比例）的二元混合物对 KS-CP 幼虫（0.95mg/L）和 DJ-CP 幼虫（1.07mg/L）的毒性明显高于花椒毒素（KS-CP 为 2.08mg/L，DJ-CP 为 2.33mg/L）或（-）-细辛脂素（11.45 和 12.61mg/L）单独使用。其他二元混合物（1:1、1:2、1:3 和 2:1 比例）和花椒毒素的毒性彼此之间没有显著差异。基于共毒系数（CC）和协同因子（SF），三种二元混合物（1:3、2:1 和 3:1）表现出协同作用（CC，250-390 和 SF，1.4-2.2，KS-CP；CC，257-279 和 SF，1.1-2.1，DJ-CP）。花椒毒素和（-）-细辛脂素的二元混合物值得进一步研究，作为控制抗杀虫剂蚊虫种群的潜在幼虫杀虫剂。
