Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
J Periodontal Res. 2012 Oct;47(5):554-62. doi: 10.1111/j.1600-0765.2011.01466.x. Epub 2012 Feb 6.
Resistin is an adipocytokine that induces insulin resistance and is predominantly expressed in adipocytes and peripheral blood mononuclear cells. Resistin expression increases in inflammatory diseases as well as diabetes mellitus, and is upregulated by bacterial pathogens and proinflammatory cytokines. The aim of this study was to identify resistin in human gingival crevicular fluid, to compare the resistin levels in gingival crevicular fluid between subjects with and without periodontitis and diabetes mellitus and to investigate the regulation of resistin release from human neutrophils by Porphyromonas gingivalis lipopolysaccharide (P-LPS).
Gingival crevicular fluid samples were collected from patients with chronic periodontitis (n = 24), patients with diabetes mellitus-related periodontitis (n = 18) and healthy subjects (n = 21). Resistin in gingival crevicular fluid was determined using western blot analysis and an ELISA kit. The glycated hemoglobin (HbA(1c)) value was obtained from patients with diabetes mellitus-related periodontitis by a medical interview. Human neutrophils were cultured with P-LPS (0-1000 ng/mL), or incubated with inhibitors of actin or microtubule polymerization in the absence or presence of P-LPS. The medium and cellular fractions were used for determination of resistin by ELISA.
The resistin level in gingival crevicular fluid from patients with periodontitis or diabetes mellitus-related periodontitis was significantly higher than that of healthy subjects. The resistin level in gingival crevicular fluid was correlated with gingival index score, but not blood HbA(1c) value. The P-LPS increased resistin release from human neutrophils, and its induction was decreased by actin polymerization inhibitors.
We show, for the first time, the presence of resistin in gingival crevicular fluid. A high resistin level in gingival crevicular fluid samples from periodontitis patients may to some extent be related to P-LPS-induced resistin release from neutrophils.
抵抗素是一种脂肪细胞因子，可诱导胰岛素抵抗，主要在脂肪细胞和外周血单核细胞中表达。抵抗素在炎症性疾病和糖尿病中表达增加，并可被细菌病原体和促炎细胞因子上调。本研究旨在鉴定人牙龈沟液中的抵抗素，比较牙周炎和糖尿病患者与健康受试者之间牙龈沟液中的抵抗素水平，并研究牙龈卟啉单胞菌脂多糖（P-LPS）对人中性粒细胞抵抗素释放的调节作用。
收集慢性牙周炎患者（n=24）、糖尿病相关牙周炎患者（n=18）和健康受试者（n=21）的牙龈沟液样本。采用 Western blot 分析和 ELISA 试剂盒检测牙龈沟液中的抵抗素。通过对糖尿病相关牙周炎患者的医疗访谈获得糖化血红蛋白（HbA（1c））值。用 P-LPS（0-1000ng/mL）培养人中性粒细胞，或在不存在或存在 P-LPS 的情况下用肌动蛋白或微管聚合抑制剂孵育。通过 ELISA 测定培养基和细胞部分中的抵抗素。
牙周炎或糖尿病相关牙周炎患者的牙龈沟液中抵抗素水平明显高于健康受试者。牙龈沟液中的抵抗素水平与牙龈指数评分相关，但与血液 HbA（1c）值无关。P-LPS 增加了人中性粒细胞抵抗素的释放，其诱导作用被肌动蛋白聚合抑制剂所抑制。
我们首次证明了抵抗素存在于牙龈沟液中。牙周炎患者牙龈沟液样本中高浓度的抵抗素在一定程度上可能与 P-LPS 诱导中性粒细胞释放抵抗素有关。
