Fujibayashi Y, Yonekura Y, Takemura Y, Wada K, Matsumoto K, Tamaki N, Yamamoto K, Konishi J, Yokoyama A
Department of Nuclear Medicine, School of Medicine, Kyoto University, Japan.
J Nucl Med. 1990 Nov;31(11):1818-22.
To clarify the relationship between the myocardial accumulation of 125I-15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) and intracellular adenosine-5'-triphosphate (ATP) content, the effect of 2,4-dinitrophenol (DNP, an electron transport uncoupler) on myocardial BMIPP accumulation was studied, in comparison with that of thallium-201-chloride (201Tl-Cl). In the mouse myocardium, DNP decreased the intracellular ATP and ADP levels, without affecting either acyl-CoA synthetase activity or the level of CoA-SH. Following treatment with DNP, decreases in myocardial BMIPP accumulation correlated well with those of ATP, while 201Tl-Cl showed slightly increased accumulation in the myocardium. Thus, in some diseases, BMIPP may be useful in evaluating myocardial ATP levels.
为阐明125I-15-（对碘苯基）-3-（R，S）-甲基十五烷酸（BMIPP）在心肌中的蓄积与细胞内三磷酸腺苷（ATP）含量之间的关系，研究了2,4-二硝基苯酚（DNP，一种电子传递解偶联剂）对心肌BMIPP蓄积的影响，并与氯化铊（201Tl-Cl）进行比较。在小鼠心肌中，DNP降低了细胞内ATP和ADP水平，但不影响酰基辅酶A合成酶活性或辅酶A-SH水平。用DNP处理后，心肌BMIPP蓄积的减少与ATP的减少密切相关，而201Tl-Cl在心肌中的蓄积略有增加。因此，在某些疾病中，BMIPP可能有助于评估心肌ATP水平。
