Wisedchanwet Trong, Wongpatcharachai Manoosak, Boonyapisitsopa Supanat, Bunpapong Napawan, Jairak Waleemas, Kitikoon Pravina, Sasipreeyajun Jiroj, Amonsin Alongkorn
Emerging and Re-emerging Infectious Diseases in Animals, Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Patumwan, Bangkok, Thailand.
Avian Dis. 2011 Dec;55(4):593-602. doi: 10.1637/9681-020811-Reg.1.
A one-year influenza A survey was conducted in 10 live bird markets (LBMs) in H5N1 high-risk areas in Thailand from January to December 2009. The result from the survey showed that the occurrence of influenza A virus (IAV) in LBMs was 0.36% (19/5304). Three influenza A subtypes recovered from LBMs were H4N6 (n = 2), H4N9 (n = 1), and H10N3 (n = 16) from Muscovy ducks housed in one LBM in Bangkok. These influenza subtypes had never been reported in Thailand, and therefore such genetic diversity raises concern about potential genetic reassortment of the viruses in avian species in a particular setting. Two influenza A subtypes (H4N6 and H4N9) were isolated from oropharyngeal and cloacal swabs of the same duck, suggesting coinfection with two influenza subtypes and possible genetic reassortment in the bird. In addition, H10N3 infection in ducks housed in the same LBM was observed. These findings further support that LBMs are a potential source of IAV transmission and genetic reassortment.
2009年1月至12月，在泰国H5N1高风险地区的10个活禽市场开展了为期一年的甲型流感调查。调查结果显示，活禽市场中甲型流感病毒（IAV）的检出率为0.36%（19/5304）。从曼谷一个活禽市场饲养的番鸭中分离出的三种甲型流感病毒亚型为H4N6（n = 2）、H4N9（n = 1）和H10N3（n = 16）。这些流感病毒亚型在泰国从未有过报道，因此这种遗传多样性引发了人们对特定环境下禽类中病毒潜在基因重配的担忧。从同一只鸭子的口咽拭子和泄殖腔拭子中分离出两种甲型流感病毒亚型（H4N6和H4N9），表明该鸭子感染了两种流感病毒亚型，并且可能在禽类中发生了基因重配。此外，还观察到同一活禽市场饲养的鸭子感染了H10N3。这些发现进一步证明，活禽市场是IAV传播和基因重配的潜在源头。
