Department of Chemistry & Biochemistry, University of California, Santa Barbara, California 93106, USA.
J Am Chem Soc. 2012 Feb 22;134(7):3346-8. doi: 10.1021/ja2115663. Epub 2012 Feb 10.
Transcription factor expression levels, which sensitively reflect cellular development and disease state, are typically monitored via cumbersome, reagent-intensive assays that require relatively large quantities of cells. Here, we demonstrate a simple, quantitative approach to their detection based on a simple, electrochemical sensing platform. This sensor sensitively and quantitatively detects its target transcription factor in complex media (e.g., 250 μg/mL crude nuclear extracts) in a convenient, low-reagent process requiring only 10 μL of sample. Our approach thus appears a promising means of monitoring transcription factor levels.
转录因子表达水平能够灵敏地反映细胞的发育和疾病状态，通常通过繁琐、试剂密集型的检测方法进行监测，这些方法需要相对大量的细胞。在这里，我们展示了一种基于简单电化学传感平台的简单、定量的检测方法。该传感器在方便、低试剂的过程中，仅需 10 μL 样品，即可灵敏且定量地检测复杂介质（例如 250μg/mL 粗核提取物）中的目标转录因子。因此，我们的方法似乎是一种有前途的监测转录因子水平的手段。
