Alagwu E A, Nneli R O, Egwurugwu J N, Osim E E
College of Medicine and Health Sciences, Imo State University, Owerri.
Niger J Physiol Sci. 2011 Nov 23;26(1):39-42.
Beneficial effect of honey has been widely reported particularly on wound healings, gastrointestinal disorders and as antibacterial agent. However, there is paucity of report on its cytoprotective effect on the gastric mucosa despite its common usage worldwide including Nigeria. This study was therefore carried out to evaluate the effect of this widely consumed substance on gastric mucosa using animal model and also to explore possible mechanism of its action on the gastric mucosa .Twenty male adult albino rats of Wistar strain, weighing between 210-220g were used in the experiment. They were randomly assigned into two groups, the control group and the honey-fed (test) group, each containing ten rats. The Control group was fed on normal rat feed and water while the test group was fed on normal rat feed with honey added to its drinking water (1ml of honey for every initial 10ml of water for each rat daily) for twenty two weeks. After twenty two weeks the rats were weighed after being starved overnight. They were anaesthetized with urethane (0.6ml/100g body weight). Gastric ulceration was induced using 1.5ml acid-alcohol prepared from equivolume of 0.1NHCl and 70% methanol introduced into the stomach via a portex cannula tied and left in place following an incision made on the antral-pyloric junction of the stomach. The acid-alcohol was allowed to stay for 1hr. After 1hr, laparatomy was performed and the stomach isolated, cut open along the greater curvature, rinsed with normal saline and fastened in place with pins on a dissecting board for ulcer examination and scores. The result obtained showed mean ulcer scores of 14.5+/-0.70 for the control group and 1.6+/-0.11 for the test group. The result showed that honey significantly reduced ulcer scores as well as caused scanty haemorrhage in the test group compared with increased ulcer scores and multiple haemorrhage in the control group. It is therefore concluded that honey intake offered cytoprotection on the gastric mucosa of albino rats.
蜂蜜的有益作用已被广泛报道，特别是在伤口愈合、胃肠道疾病以及作为抗菌剂方面。然而，尽管蜂蜜在包括尼日利亚在内的全球范围内广泛使用，但关于其对胃黏膜细胞保护作用的报道却很少。因此，本研究使用动物模型评估这种广泛食用的物质对胃黏膜的影响，并探讨其对胃黏膜作用的可能机制。实验选用了20只体重在210 - 220克之间的成年雄性Wistar白化大鼠。它们被随机分为两组，即对照组和蜂蜜喂养（试验）组，每组各10只大鼠。对照组给予正常大鼠饲料和水，而试验组给予添加了蜂蜜的正常大鼠饲料（每只大鼠每天最初的10毫升水中添加1毫升蜂蜜），持续22周。22周后，大鼠禁食过夜后称重。用乌拉坦（0.6毫升/100克体重）麻醉。通过在胃窦 - 幽门交界处切开后插入并固定的波特克斯套管，将由等体积的0.1N盐酸和70%甲醇配制而成的1.5毫升酸 - 乙醇注入胃内，诱导胃溃疡形成。酸 - 乙醇在胃内停留1小时。1小时后，进行剖腹手术，分离出胃，沿大弯切开，用生理盐水冲洗，并用大头针固定在解剖板上进行溃疡检查和评分。结果显示，对照组的平均溃疡评分为14.5±0.70，试验组为1.6±0.11。结果表明，与对照组溃疡评分增加和多处出血相比，蜂蜜显著降低了试验组的溃疡评分，且出血较少。因此得出结论，摄入蜂蜜对白化大鼠的胃黏膜具有细胞保护作用。
