Cui Li-Jin, Zhang Chen, Zhang Ting, Lu Rou-Jian, Xie Zheng-De, Zhang Ling-Lin, Liu Chuan-Yan, Zhou Wei-Min, Ruan Li, Ma Xue-Jun, Tan Wen-Jie
National Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
Adv Virol. 2011;2011:129134. doi: 10.1155/2011/129134. Epub 2011 Jul 21.
The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation.
人冠状病毒（HCoVs）HCoV-NL63和HCoV-HKU1是最近发现的两种冠状病毒，广泛传播并与急性呼吸道感染（ARI）相关。我们在北京儿童医院收集了2008年5月至2010年3月期间年龄小于7.75岁的ARI患者的标本，检测其中的HCoV-NL63和HCoV-HKU1。通过实时逆转录聚合酶链反应（RT-PCR），382份标本中分别有32份（8.4%）和57份（14.9%）的HCoV-NL63和HCoV-HKU1检测呈阳性。使用Luminex xTAG RVP Fast试剂盒检测显示，呼吸道合胞病毒和副流感3型病毒的共同感染在感染这两种病毒类型的患者中很常见。在感染HCoV-HKU1的患者中，主要临床症状为咳嗽、发热和咳痰。在感染HCoV-NL63的患者中，主要症状为咳嗽、发热和流涕。系统发育研究表明，与美国国立生物技术信息中心（NCBI）参考序列相比，HCoV-HKU1核蛋白基因相对保守，而HCoV-NL63的1ab基因显示出更多变异。
