Center of Research and Innovation on Sports, University Claude Bernard Lyon 1, University of Lyon, Villeurbanne, France.
J Appl Physiol (1985). 2012 May;112(9):1445-53. doi: 10.1152/japplphysiol.01452.2011. Epub 2012 Feb 9.
The aim of this study was to analyze the effects of exercise training on oxidative stress in sickle cell trait carriers. Plasma levels of oxidative stress [advanced oxidation protein products (AOPP), protein carbonyl, malondialdehyde (MDA), and nitrotyrosine], antioxidant markers [catalase, glutathione peroxidase (GPX), and superoxide dismutase (SOD)], and nitrite and nitrate (NOx) were assessed at baseline, immediately following a maximal exercise test (T(ex)), and during recovery (T(1h), T(2h), T(24h)) in trained (T: 8 h/wk minimum) and untrained (U: no regular physical activity) sickle cell trait (SCT) carriers or control (CON) subjects (T-SCT, n = 10; U-SCT, n = 8; T-CON, n = 11; and U-CON, n = 11; age: 23.5 ± 2.2 yr). The trained subjects had higher SOD activities (7.6 ± 5.4 vs. 5.2 ± 2.1 U/ml, P = 0.016) and lower levels of AOPP (142 ± 102 vs. 177 ± 102 μM, P = 0.028) and protein carbonyl (82.1 ± 26.0 vs. 107.3 ± 30.6 nm/ml, P = 0.010) than the untrained subjects in response to exercise. In response to exercise, U-SCT had a higher level of AOPP (224 ± 130 vs. 174 ± 121 μM, P = 0.012), nitrotyrosine (127 ± 29.1 vs.70.6 ± 46.6 nM, P = 0.003), and protein carbonyl (114 ± 34.0 vs. 86.9 ± 26.8 nm/ml, P = 0.006) compared with T-SCT. T-SCT had a higher SOD activity (8.50 ± 7.2 vs. 4.30 ± 2.5 U/ml, P = 0.002) and NOx (28.8 ± 11.4 vs. 14.6 ± 7.0 μmol·l(-1)·min(-1), P = 0.003) in response to exercise than U-SCT. Our data indicate that the overall oxidative stress and nitric oxide response is improved in exercise-trained SCT carriers compared with their untrained counterparts. These results suggest that physical activity could be a viable method of controlling the oxidative stress. This could have a beneficial impact because of its involvement in endothelial dysfunction and subsequent vascular impairment in hemoglobin S carriers.
本研究旨在分析运动训练对镰状细胞特质携带者氧化应激的影响。在训练（T：每周至少 8 小时）和未训练（U：无规律体育活动）的镰状细胞特质（SCT）携带者或对照组（T-SCT，n=10；U-SCT，n=8；T-CON，n=11；和 U-CON，n=11；年龄：23.5±2.2 岁）中，在基线时、最大运动测试后即刻（T(ex)）以及恢复期间（T(1h)、T(2h)、T(24h)）评估了氧化应激 [氧化蛋白产物（AOPP）、蛋白羰基、丙二醛（MDA）和硝基酪氨酸]、抗氧化标志物 [过氧化氢酶、谷胱甘肽过氧化物酶（GPX）和超氧化物歧化酶（SOD）]以及亚硝酸盐和硝酸盐（NOx）的水平。与未训练组相比，训练组的 SOD 活性更高（7.6±5.4 比 5.2±2.1 U/ml，P=0.016），AOPP（142±102 比 177±102 μM，P=0.028）和蛋白羰基（82.1±26.0 比 107.3±30.6 nm/ml，P=0.010）水平更低。与 T-SCT 相比，U-SCT 在运动后 AOPP（224±130 比 174±121 μM，P=0.012）、硝基酪氨酸（127±29.1 比 70.6±46.6 nM，P=0.003）和蛋白羰基（114±34.0 比 86.9±26.8 nm/ml，P=0.006）的水平更高。与 U-SCT 相比，T-SCT 在运动后 SOD 活性更高（8.50±7.2 比 4.30±2.5 U/ml，P=0.002）和 NOx 更高（28.8±11.4 比 14.6±7.0 μmol·l(-1)·min(-1)，P=0.003）。我们的数据表明，与未训练的 SCT 携带者相比，运动训练的 SCT 携带者的整体氧化应激和一氧化氮反应得到改善。这些结果表明，体育活动可能是控制氧化应激的可行方法。由于它涉及血红蛋白 S 携带者的内皮功能障碍和随后的血管损伤，因此这可能具有有益的影响。
