College of Life Sciences, Henan University, Kaifeng 475100, China.
Plant Sci. 2012 Apr;185-186:176-84. doi: 10.1016/j.plantsci.2011.10.007. Epub 2011 Oct 14.
Thurber's cotton (Gossypium thurberi) is the wild relative of cultivated cotton. It is highly resistant to cotton Verticillium wilt, a disease that significantly affects cotton yield and quality. To reveal the mechanism of disease resistance in G. thurberi and to clone resistance-related genes, we used two-dimensional electrophoresis (2-DE) and tandem time-of-flight mass spectrometry (MALDI-TOF-MS) to identify differentially expressed proteins in Thurber's cotton after inoculation with Verticillium dahliae. A total of 57 different protein spots were upregulated, including 52 known proteins representing 11% of the total protein spots. These proteins are involved in resistance to stress and disease, transcriptional regulation, signal transduction, protein processing and degradation, photosynthesis, production capacity, basic metabolism, and other processes. In addition, five disease resistance proteins showed intense upregulation, indicating that resistance genes (R genes) may play a critical role in resistance to Verticillium wilt in Thurber's cotton. Our results suggest that disease and stress resistance are the combined effects of multiple co-expressed genes. This provides a basis for further, detailed investigation into the mechanisms underlying Verticillium wilt resistance of G. thurberi and for cloning essential genes into cotton cultivars to produce Verticillium wilt resistant plants.
瑟伯氏棉（Gossypium thurberi）是栽培棉的野生近缘种。它对棉花黄萎病具有高度抗性，这种病会显著影响棉花的产量和品质。为了揭示瑟伯氏棉的抗病机制并克隆抗性相关基因，我们使用二维电泳（2-DE）和串联时间飞行质谱（MALDI-TOF-MS）技术，在接种大丽轮枝菌后鉴定出瑟伯氏棉中的差异表达蛋白。共有 57 个不同的蛋白斑点被上调，其中包括 52 种已知蛋白，占总蛋白斑点的 11%。这些蛋白参与了对胁迫和疾病的抗性、转录调控、信号转导、蛋白加工和降解、光合作用、产能、基础代谢和其他过程。此外，五种抗病蛋白表现出强烈的上调，表明抗病基因（R 基因）可能在瑟伯氏棉抗黄萎病中发挥关键作用。我们的结果表明，疾病和胁迫抗性是多个共表达基因的综合作用。这为进一步深入研究瑟伯氏棉抗黄萎病的机制以及将关键基因克隆到棉花品种中生产抗黄萎病的植株提供了基础。
