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呼吸爆发氧化酶同源蛋白 D()正向调控棉花对 的抗性。

The Respiratory Burst Oxidase Homolog Protein D () Positively Regulates the Cotton Resistance to .

机构信息

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Life Science, Zhengzhou University, Zhengzhou 450001, China.

出版信息

Int J Mol Sci. 2021 Dec 2;22(23):13041. doi: 10.3390/ijms222313041.

Abstract

Verticillium wilt, mainly caused by a soil-inhabiting fungus , can seriously reduce the yield and quality of cotton. The complex mechanism underlying cotton resistance to Verticillium wilt remains largely unknown. In plants, reactive oxygen species (ROS) mediated by Rbohs is one of the earliest responses of plants to biotic and abiotic stresses. In our previous study, we performed a time-course phospho-proteomic analysis of roots of resistant and susceptible cotton varieties in response to , and found early differentially expressed protein burst oxidase homolog protein D (). However, the role of -mediated ROS in cotton defense against needs further investigation. In this study, we analyzed the function of -mediated resistance of cotton against in vitro and in vivo. Bioinformatics analysis showed that GhRbohD possessed the conservative structural attributes of Rbohs family, 12 members of RbohD out of 57 Rbohs in cotton. The expression of was significantly upregulated after inoculation, peaking at 6 hpi, and the phosphorylation level was also increased. A VIGS test demonstrated that ROS production, NO, HO and Ca contents of -silenced cotton plants were significantly reduced, and lignin synthesis and callose accumulation were damaged, important reasons for the impairment of -silenced cotton's defense against . The expression levels of resistance-related genes were downregulated in -silenced cotton by qRT-PCR, mainly involving the lignin metabolism pathway and the jasmonic acid signaling pathway. However, overexpression of enhanced resistance of transgenic to challenge. Furthermore, Y2H assays were applied to find that GhPBL9 and GhRPL12C may interact with GhRbohD. These results strongly support that activates ROS production to positively regulate the resistance of plants against .

摘要

黄萎病主要由土壤栖居真菌引起,可严重降低棉花的产量和品质。棉花对黄萎病抗性的复杂机制在很大程度上尚不清楚。在植物中,由 Rbohs 介导的活性氧 (ROS) 是植物对生物和非生物胁迫的最早反应之一。在我们之前的研究中,我们对感病和抗病棉花品种的根系进行了时间过程磷酸化蛋白质组分析,以响应并发现早期差异表达的蛋白爆氧化还原酶同源蛋白 D ()。然而,-介导的 ROS 在棉花防御中的作用需要进一步研究。在这项研究中,我们分析了 -介导的棉花对黄萎病的抗性在体外和体内的功能。生物信息学分析表明,GhRbohD 具有 Rbohs 家族的保守结构属性,在棉花的 57 个 Rbohs 中,有 12 个属于 RbohD。接种后GhRbohD 的表达显著上调,在 6 hpi 时达到峰值,磷酸化水平也增加。VIGS 试验表明,-沉默棉花植物的 ROS 产生、NO、HO 和 Ca 含量显著降低,木质素合成和胼胝质积累受损,这是 -沉默棉花防御功能受损的重要原因。通过 qRT-PCR 检测,-沉默棉花中抗性相关基因的表达水平下调,主要涉及木质素代谢途径和茉莉酸信号通路。然而,过表达GhRbohD 增强了转基因对黄萎病挑战的抗性。此外,Y2H 测定用于发现 GhPBL9 和 GhRPL12C 可能与 GhRbohD 相互作用。这些结果强烈支持 GhRbohD 通过激活 ROS 产生来正向调节植物对的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10f7/8657740/9dc84d4ac177/ijms-22-13041-g001.jpg

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