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稀酸预处理桉木片的酶糖化生产可发酵糖。

Enzymatic saccharification of dilute acid pretreated eucalyptus chips for fermentable sugar production.

机构信息

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.

出版信息

Bioresour Technol. 2012 Apr;110:302-7. doi: 10.1016/j.biortech.2012.01.003. Epub 2012 Jan 9.

DOI:10.1016/j.biortech.2012.01.003
PMID:22325899
Abstract

Dilute sulfuric acid was used to pretreat eucalyptus chips prior to enzymatic hydrolysis. After both pretreatment and enzymatic hydrolysis processes. Attention is paid to sugar recovery. The maximum total sugars yield (combined xylose and glucose, 47.69g/100g raw material, representing 82% of total sugars in the eucalyptus biomass) was obtained at 160°C, 0.75% acid concentration and 10min residence time, which is consider to be the best reasonable conditions for the dilute acid pretreatment of eucalyptus, corresponding concentrations of acetic acid, HMF, and furfural in the prehydrolysate were about 2.01g/L, 0.13g/L and 1.37g/L, respectively. Under this optimal pretreatment condition, the acid-insoluble lignin recovery in the insoluble solid resulting from enzymatic hydrolysis, was 22.7g/100g raw material, representing 80% of acid-insoluble lignin in the eucalyptus biomass.

摘要

稀硫酸被用于预处理桉木片,然后进行酶水解。在预处理和酶水解过程之后,要注意糖的回收。在 160°C、0.75%酸浓度和 10 分钟保留时间下,获得了最大总糖得率(结合木糖和葡萄糖,为 47.69g/100g 原料,占桉树木质素中总糖的 82%),这被认为是桉木稀酸预处理的最佳合理条件,在预水解物中对应的乙酸、HMF 和糠醛浓度分别约为 2.01g/L、0.13g/L 和 1.37g/L。在这种最佳预处理条件下,酶水解后不溶性固体中酸不溶性木质素的回收量为 100g 原料 22.7g,占桉树木质素中酸不溶性木质素的 80%。

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