Ecological Department of Water Problems Institute, Russian Academy of Sciences, 3 Gubkina Str., Moscow 119333, Russian Federation.
Bioresour Technol. 2012 Apr;110:706-10. doi: 10.1016/j.biortech.2012.01.058. Epub 2012 Jan 25.
A change in δ(13)CH(4) during mesophilic methanization of cellulosic waste (paper and cardboard) was described using a mathematical model based on stoichiometric chemical reactions, microbial dynamics and the equation for the (13)C isotope accumulation in products including isotope fractionation. In this study, experimental data, previously obtained by Qu et al. (2009), was used to model metabolic pathways of cellulose transformation. A significant change in δ(13)CH(4) occurred in time during cellulosic waste methanization which was in accordance with the model. It was explained by the change in input of acetoclastic and hydrogenotrophic methanogenesis as well as by fractionation of stable carbon isotopes (13)C and (12)C which was much higher for hydrogenotrophic methanogenesis when compared to acetoclastic methanogenesis.
使用基于化学计量学反应、微生物动力学和产物中（13）C 同位素积累方程（包括同位素分馏）的数学模型，描述了纤维素废物（纸和纸板）在中温甲烷化过程中 δ(13)CH(4) 的变化。在这项研究中，使用了 Qu 等人（2009 年）先前获得的实验数据来模拟纤维素转化的代谢途径。在纤维素废物甲烷化过程中，δ(13)CH(4) 在时间上发生了显著变化，这与模型相符。这可以通过乙酰营养型和氢营养型甲烷生成的输入变化以及稳定碳同位素（13）C 和（12）C 的分馏来解释，与乙酰营养型甲烷生成相比，氢营养型甲烷生成的（13）C 和（12）C 分馏要高得多。
