Flynn Thomas R, Paster Bruce J, Stokes Lauren N, Susarla Srinivas M, Shanti Rabie M
Department of Oral and Maxillofacial Surgery, Harvard School of Dental Medicine, Boston, MA, USA.
J Oral Maxillofac Surg. 2012 Aug;70(8):1854-9. doi: 10.1016/j.joms.2011.09.009. Epub 2012 Feb 10.
Historically, the identification of microorganisms has been limited to species that could be cultured in the microbiology laboratory. The purpose of the present study was to apply molecular techniques to identify microorganisms in orofacial odontogenic infections (OIs).
Specimens were obtained from subjects with clinical evidence of OI. To identify the microorganisms involved, 16S rRNA sequencing methods were used on clinical specimens. The name and number of the clones of each species identified and the combinations of species present were recorded for each subject. Descriptive statistics were computed for the study variables.
Specimens of pus or wound fluid were obtained from 9 subjects. A mean of 7.4 ± 3.7 (standard deviation) species per case were identified. The predominant species detected in the present study that have previously been associated with OIs were Fusobacterium spp, Parvimonas micra, Porphyromonas endodontalis, and Prevotella oris. The predominant species detected in our study that have not been previously associated with OIs were Dialister pneumosintes and Eubacterium brachy. Unculturable phylotypes accounted for 24% of the species identified in our study. All species detected were obligate or facultative anaerobes. Streptococci were not detected.
Molecular methods have enabled us to detect previously cultivated and not-yet-cultivated species in OIs; these methods could change our understanding of the pathogenic flora of orofacial OIs.
在历史上，微生物的鉴定仅限于那些能够在微生物实验室中培养的物种。本研究的目的是应用分子技术来鉴定口腔颌面部牙源性感染（OI）中的微生物。
从有OI临床证据的受试者中获取标本。为了鉴定所涉及的微生物，对临床标本采用16S rRNA测序方法。记录每个受试者鉴定出的每种物种的克隆名称和数量以及存在的物种组合。对研究变量进行描述性统计。
从9名受试者中获取了脓液或伤口液体标本。每例平均鉴定出7.4±3.7（标准差）种物种。在本研究中检测到的先前与OI相关的主要物种为梭杆菌属、微小单胞菌、牙髓卟啉单胞菌和口普雷沃菌。在我们的研究中检测到的先前未与OI相关的主要物种为肺炎戴阿利斯特菌和短真杆菌。不可培养的系统发育型占我们研究中鉴定出的物种的24%。检测到的所有物种均为专性或兼性厌氧菌。未检测到链球菌。
分子方法使我们能够在OI中检测到先前已培养和尚未培养的物种；这些方法可能会改变我们对口面部OI致病菌群的认识。
