Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
Gubkin Russian State University of Oil and Gas, Leninskiy Prospect 65, 117485 Moscow, Russia.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):86-92. doi: 10.1099/ijs.0.041012-0. Epub 2012 Feb 10.
A novel obligately anaerobic, mesophilic, organotrophic bacterium, strain P3M-1(T), was isolated from a microbial mat formed in a wooden bath filled with hot water emerging from a 2775 m-deep well in the Tomsk region of western Siberia, Russia. Cells of strain P3M-1(T) were rod-shaped, 0.3-0.7 µm in width and formed multicellullar filaments that reached up to 400 µm in length. Strain P3M-1(T) grew optimally at 42-45 °C, pH 7.5-8.0, and with 0.1% (w/v) NaCl. Under optimal conditions, the doubling time was 6 h. The isolate was able to ferment a variety of proteinaceous substrates and sugars, including microcrystalline cellulose. Acetate, ethanol and H(2) were the main products of glucose fermentation. The genomic DNA G+C content was 55 mol%. 16S rRNA gene sequence-based phylogenetic analyses showed that strain P3M-1(T) was a member of the class Anaerolinea, with 92.8 % sequence similarity to Levilinea saccharolytica KIBI-1(T). Based on phylogenetic analysis and physiological properties, strain P3M-1(T) represents a novel species in a new genus, for which the name Ornatilinea apprima gen. nov., sp. nov. is proposed; the type strain of O. apprima is P3M-1(T) (= DSM 23815(T)=VKM B-2669(T)).
一株新型专性厌氧、中温、有机营养型细菌，菌株 P3M-1(T)，从俄罗斯西伯利亚西部托木斯克地区一口 2775 米深的热水井中木制浴盆里形成的微生物垫中分离得到。P3M-1(T)菌株的细胞呈杆状，宽 0.3-0.7μm，形成长达 400μm的多细胞丝。P3M-1(T)菌株在 42-45°C、pH7.5-8.0 和 0.1%（w/v）NaCl 条件下生长最佳。在最佳条件下，倍增时间为 6 小时。该分离株能够发酵多种蛋白质和糖底物，包括微晶纤维素。葡萄糖发酵的主要产物是乙酸盐、乙醇和 H₂。基因组 DNA G+C 含量为 55mol%。基于 16S rRNA 基因序列的系统发育分析表明，P3M-1(T)菌株属于 Anaerolineae 类，与 Levilinea saccharolytica KIBI-1(T)的序列相似性为 92.8%。基于系统发育分析和生理特性，P3M-1(T)菌株代表了一个新属中的一个新种，提议将其命名为 Ornatilinea apprima gen. nov., sp. nov.；O. apprima 的模式株为 P3M-1(T)（=DSM 23815(T)=VKM B-2669(T)）。
