Ehn Andreas, Johansson Olof, Arvidsson Andreas, Aldén Marcus, Bood Joakim
Division of Combustion Physics, Lund University, Box 118, SE-221 00 Lund, Sweden.
Opt Express. 2012 Jan 30;20(3):3043-56. doi: 10.1364/OE.20.003043.
A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of ~120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques. Monte Carlo simulations were conducted to analyze the sensitivity of the detector scheme as well as to compare the proposed evaluation algorithm to previously presented rapid lifetime determination algorithms.
本文提出了一种用于评估单激光脉冲荧光寿命的新技术，称为双成像与建模评估（DIME）。该技术在一个通用气体混合实验中得到了实验验证，以清晰展示探测器方案的快速性和灵敏度。确定了单激光脉冲荧光寿命约为800 ps，标准偏差约为120 ps。这些结果与条纹相机测量结果进行了比较。此外，还提出了一种通用的荧光寿命测定算法。该评估算法在荧光寿命与探测器信号比之间具有解析线性关系。与DIME探测器方案相结合，它是一种比先前提出的技术更快、更准确、更灵敏的快速荧光寿命成像方法。进行了蒙特卡罗模拟，以分析探测器方案的灵敏度，并将所提出的评估算法与先前提出的快速寿命测定算法进行比较。
