Department of Animal Sciences, University of Florida, PO Box 110910, Gainesville, FL, USA.
Reprod Biol Endocrinol. 2012 Feb 13;10:12. doi: 10.1186/1477-7827-10-12.
Bone morphogenetic proteins (BMPs) were first described for their roles in bone formation, but they now also are known to possess additional activities, including those relating to embryogenesis. The objectives of this work were to 1) determine if peri-attachment bovine conceptuses and bovine trophoblast cells (CT1) contain transcripts for BMP2 and 4, an innate inhibitor noggin (NOG), and BMP2/4 receptors (BMPRII, ACVR1, BMPR1A, BMPR1B), and 2) determine if BMP2 or 4 supplementation to CT1 cells affects cell proliferation, differentiation or trophoblast-specific gene expression.
RNA was isolated from day 17 bovine conceptuses and CT1 cells. After RT-PCR, amplified products were cloned and sequenced. In other studies CT1 cells were treated with BMP2 or 4 at various concentrations and effects on cell viability, cell differentiation and abundance of IFNT and CSH1 mRNA were evaluated.
Transcripts for BMP2 and 4 were detected in bovine conceptuses and CT1 cells. Also, transcripts for each BMP receptor were detected in conceptuses and CT1 cells. Transcripts for NOG were detected in conceptuses but not CT1 cells. Cell proliferation was reduced by BMP4 but not BMP2 supplementation. Both factors reduced IFNT mRNA abundance but had no effect on CSH1 mRNA abundance in CT1 cells.
The BMP2/4 ligand and receptor system presides within bovine trophectoderm prior to uterine attachment. BMP4 negatively impacts CT1 cell growth and both BMPs affect IFNT mRNA abundance.
骨形态发生蛋白（BMPs）最初因其在骨形成中的作用而被描述，但现在也已知其具有额外的活性，包括与胚胎发生有关的活性。本研究的目的是：1）确定附着前的牛胚胎和牛滋养层细胞（CT1）是否含有 BMP2 和 4 的转录本、天然抑制剂 noggin（NOG）以及 BMP2/4 受体（BMPRII、ACVR1、BMPR1A、BMPR1B），2）确定 BMP2 或 4 对 CT1 细胞的补充是否影响细胞增殖、分化或滋养层特异性基因表达。
从第 17 天的牛胚胎和 CT1 细胞中分离 RNA。经过 RT-PCR 后，扩增产物被克隆和测序。在其他研究中，用不同浓度的 BMP2 或 4 处理 CT1 细胞，评估对细胞活力、细胞分化以及 IFNT 和 CSH1 mRNA 丰度的影响。
在牛胚胎和 CT1 细胞中检测到 BMP2 和 4 的转录本。此外，在胚胎和 CT1 细胞中也检测到每个 BMP 受体的转录本。在胚胎中检测到 NOG 的转录本，但在 CT1 细胞中未检测到。BMP4 而非 BMP2 的补充会降低细胞增殖。两种因子均降低 CT1 细胞中 IFNT mRNA 的丰度，但对 CSH1 mRNA 的丰度没有影响。
在附着前的牛滋养层中存在 BMP2/4 配体和受体系统。BMP4 负向影响 CT1 细胞的生长，两种 BMP 均影响 IFNT mRNA 的丰度。
