Shih T Y, Weeks M O, Young H A, Scholnick E M
Laboratory of Tumor Virus Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205, USA.
Virology. 1979 Jul 15;96(1):64-79. doi: 10.1016/0042-6822(79)90173-9.
A similar protein of 21,000 MW (p21) coded for by Harvey or Kirsten murine sarcoma virus has been identified in nonproducer cells transformed by these two viruses. Antisera prepared from rats bearing tumors induced by syngeneic transplantation of NRK cells transformed by Harvey murine sarcoma virus (Ha-MuSV) specifically precipitated the Ha-MuSV p21 from a nonproducer Balb/c mouse cell and a nonproducer dog cell transformed by Ha-MuSV. The same antisera also precipitated a similar protein, Ki-MuSV p21, from a nonproducer mink cell transformed by Kirsten murine sarcoma virus (Ki-MuSV). Both the p21 of Ha-MuSV and of Ki-MuSV are phosphoproteins. Previous studies have reported a virus-specific p21 polypeptide from translation of Ha-MuSV RNA in cell-free protein synthesis systems (W. P. Parks and E. M. Scolnick, 1977, J. Virol. 22, 711-719; T. Y. Shih, D. R. Williams, M. O. Weeks, J. M. Maryak, W. C. Vass, and E. M. Scolnick, 1978, J. Virol 27, 45-55). This p21 protein was specifically precipitated by the same anti-tumor sera. Similarly, a p21 polypeptide translated from Ki-MuSV RNA was also specifically precipitated by the antitumor sera. Therefore, it is concluded that the p21 of Ha-MuSV and Ki-MuSV are homologous proteins coded for bv homologous sequences found in the recombinant genomes of Ha-MuSV and Ki-MuSV.
哈维或克里斯滕鼠肉瘤病毒编码的一种分子量为21,000的类似蛋白质（p21）已在被这两种病毒转化的非生产性细胞中被鉴定出来。用哈维鼠肉瘤病毒（Ha-MuSV）转化的NRK细胞经同基因移植诱导产生肿瘤的大鼠制备的抗血清，能从一个非生产性Balb/c小鼠细胞和一个被Ha-MuSV转化的非生产性犬细胞中特异性沉淀出Ha-MuSV p21。同样的抗血清也能从一个被克里斯滕鼠肉瘤病毒（Ki-MuSV）转化的非生产性貂细胞中沉淀出一种类似的蛋白质，即Ki-MuSV p21。Ha-MuSV和Ki-MuSV的p21都是磷蛋白。先前的研究报道了在无细胞蛋白质合成系统中由Ha-MuSV RNA翻译产生的一种病毒特异性p21多肽（W.P.帕克斯和E.M.斯科尔尼克，1977年，《病毒学杂志》22卷，711 - 719页；T.Y.施、D.R.威廉姆斯、M.O.威克斯、J.M.马里亚克、W.C.瓦斯和E.M.斯科尔尼克，1978年，《病毒学杂志》27卷，45 - 55页）。这种p21蛋白能被相同的抗肿瘤血清特异性沉淀。同样，由Ki-MuSV RNA翻译产生的p21多肽也能被抗肿瘤血清特异性沉淀。因此，可以得出结论，Ha-MuSV和Ki-MuSV的p21是同源蛋白，由在Ha-MuSV和Ki-MuSV重组基因组中发现的同源序列编码。
