柯斯顿鼠肉瘤病毒的p21在对维持转化敏感的病毒突变体温度下是热不稳定的。
p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation.
作者信息
Shih T Y, Weeks M O, Young H A, Scolnick E M
出版信息
J Virol. 1979 Aug;31(2):546-6. doi: 10.1128/JVI.31.2.546-546.1979.
Abstract
We have recently described an intracellular protein, p21, in nonproducer cells transformed by either the Kirsten (Ki-MSV) or Harvey (Ha-MSV) strain of murine sarcoma virus (Shih et al., Virology, in press). The p21 is phosphorylated and has been shown to be coded for by either Ki-MSV or Ha-MSV. In this report, we compare the thermal stability of the newly synthesized [35S]methionine-labeled p21 in cells transformed by the wild-type Ki-MSV or by a mutant of Ki-MSV (ts 371) which is temperature sensitive in a viral function required for the maintenance of several properties of the transformed phenotype. The immunoprecipitability of the p21 coded for by the ts 371 Ki-MSV was markedly more thermolabile than the p21 of the wild-type Ki-MSV when the cell extracts are heated in vitro. The present finding suggests that the p21 is required for the maintenance of transformation induced by Ki-MSV.
摘要
我们最近在由 Kirsten（Ki-MSV）或 Harvey（Ha-MSV）株鼠肉瘤病毒转化的非生产性细胞中描述了一种细胞内蛋白 p21（Shih 等人，《病毒学》，即将发表）。p21 被磷酸化，并且已证明由 Ki-MSV 或 Ha-MSV 编码。在本报告中，我们比较了在由野生型 Ki-MSV 或 Ki-MSV 的突变体（ts 371）转化的细胞中，新合成的 [35S]甲硫氨酸标记的 p21 的热稳定性，该突变体在维持转化表型的几种特性所需的病毒功能中对温度敏感。当体外加热细胞提取物时，ts 371 Ki-MSV 编码的 p21 的免疫沉淀性明显比野生型 Ki-MSV 的 p21 更不耐热。目前的发现表明，p21 是维持 Ki-MSV 诱导的转化所必需的。
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本文引用的文献
1
Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.
J Bacteriol. 1966 Oct;92(4):1133-40. doi: 10.1128/jb.92.4.1133-1140.1966.
2
Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles.
Proc Natl Acad Sci U S A. 1966 Apr;55(4):780-6. doi: 10.1073/pnas.55.4.780.
3
Temperature-dependent expression of transformation by cold-sensitive mutant of murine sarcoma virus.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2206-10. doi: 10.1073/pnas.70.8.2206.
4
Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information.
J Virol. 1974 Jun;13(6):1211-9. doi: 10.1128/JVI.13.6.1211-1219.1974.
5
Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures.
Int J Cancer. 1970 Jul 15;6(1):95-107. doi: 10.1002/ijc.2910060114.
6
Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
J Virol. 1969 Nov;4(5):549-53. doi: 10.1128/JVI.4.5.549-553.1969.
7
Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus.
J Virol. 1973 Sep;12(3):458-63. doi: 10.1128/JVI.12.3.458-463.1973.
8
RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA.
Virology. 1973 Feb;51(2):370-82. doi: 10.1016/0042-6822(73)90436-4.
9
Isolation of temperature-sensitive mutants of murine sarcoma virus.
J Virol. 1972 Oct;10(4):653-7. doi: 10.1128/JVI.10.4.653-657.1972.
10
Plaque assay techniques for murine leukemia viruses.
Virology. 1970 Dec;42(4):1136-9. doi: 10.1016/0042-6822(70)90362-4.
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