Shin K, Yamauchi K, Teraguchi S, Hayasawa H, Tomita M, Otsuka Y, Yamazaki S
Nutritional Science Laboratory, Morinaga Milk Industry Co. Ltd, Kanagawa, Japan.
Lett Appl Microbiol. 1998 Jun;26(6):407-11. doi: 10.1046/j.1472-765x.1998.00358.x.
The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 micrograms ml-1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 micrograms ml-1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli O157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides.
研究了牛乳铁蛋白（bLF）、其胃蛋白酶水解产物（bLFH）和活性肽乳铁素B（LFcinB）对4株肠出血性大肠杆菌O157:H7临床分离株的抗菌活性。在1%细菌蛋白胨肉汤中，bLF对这些分离株的最低抑菌浓度（MIC）为3 mg/ml，bLFH为0.1 - 0.2 mg/ml，LFcinB为8 - 10 μg/ml。浓度高于10 μg/ml时，LFcinB在3小时内可杀死这些细菌。透射电子显微镜观察结果表明，LFcinB作用于细菌表面并影响细胞质内容物。结果显示，LFcinB可影响大肠杆菌O157:H7菌株培养上清液中志贺毒素的水平。这些结果表明，大肠杆菌O157:H7菌株对bLF及其肽的抗菌作用敏感。
