在东南亚的静脉注射吸毒者中，B'、C 和 CRF01_AE 之间存在广泛而复杂的 HIV-1 重组。

Extensive and complex HIV-1 recombination between B', C and CRF01_AE among IDUs in south-east Asia.

机构信息

Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, and The Second People's Hospital, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan, China.

出版信息

AIDS. 2012 Jun 1;26(9):1121-9. doi: 10.1097/QAD.0b013e3283522c97.

DOI:10.1097/QAD.0b013e3283522c97

PMID:22333750

Abstract

OBJECTIVE

To investigate the subtype characterization of HIV-1 among IDUs in northern Myanmar.

DESIGN

A molecular epidemiological investigation was conducted among IDUs in Laza and Maizayang cities of northern Myanmar.

METHODS

A total of 83 HIV-1-positive serums were collected from consenting IDUs during June to August 2009. HIV-1 p17, pol, vif-env, C2V3 fragments were amplified and sequenced. Phylogenetic and bootscanning analyses were performed.

RESULTS

A very high proportion (86.1%) of HIV-1 intersubtype recombinants and very low proportion of subtypes B' (3.8%), C (7.6%) and CRF01_AE (1.3%) were found in this HIV-infected IDUs cohort. These recombinants cover all four kinds of recombination forms formed among CRF01_AE, B and C. The B/C and CRF01_AE/B/C recombinants are the two most dominant recombinants, accounting for 54.4 and 42.6% of all cases, respectively, and indicating the ongoing generation of extensive and complex HIV-1 recombination among CRF01_AE, B' and C in northern Myanmar. Intriguingly, most recombinants have different chimeric patterns from each other, forming 64 unique recombination forms (URFs) that are quite distinct from any previously identified circulating recombinant forms (CRFs) and URFs in Asia.

CONCLUSION

The extremely high proportion of intersubtype recombinants, especially CRF01_AE/B'/C recombinants (42.6%), strongly suggests that northern Myanmar is a big forge for HIV-1 recombination among CRF01_AE, B' and C.

摘要

目的

调查缅甸北部 IDU 人群中 HIV-1 的亚型特征。

设计

在缅甸北部腊戍和迈扎央市对 IDU 进行了分子流行病学调查。

方法

2009 年 6 月至 8 月，从同意的 IDU 中收集了总共 83 份 HIV-1 阳性血清。扩增并测序了 HIV-1 p17、pol、vif-env、C2V3 片段。进行了系统发育和启动扫描分析。

结果

在所研究的 HIV-1 感染 IDU 队列中，发现 HIV-1 亚型重组比例非常高（86.1%），亚型 B'（3.8%）、C（7.6%）和 CRF01_AE（1.3%）的比例非常低。这些重组体涵盖了在 CRF01_AE、B 和 C 之间形成的所有四种重组形式。B/C 和 CRF01_AE/B/C 重组体是两种最主要的重组体，分别占所有病例的 54.4%和 42.6%，表明 CRF01_AE、B'和 C 之间正在发生广泛而复杂的 HIV-1 重组。有趣的是，大多数重组体彼此之间具有不同的嵌合模式，形成 64 种独特的重组形式（URF），与亚洲已鉴定的任何循环重组形式（CRF）和 URF 都截然不同。