Anagnostopoulou Maria A, Kefalas Panagiotis
Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
Biomed Chromatogr. 2012 Oct;26(10):1252-68. doi: 10.1002/bmc.2687. Epub 2012 Feb 15.
High-performance liquid chromatography with confirmation by UV-visible photodiode array detector-positive electrospray ionization-mass spectrometry [HPLC-UV-vis-DAD-(+ESI)-MS] with enhanced fragmentation by appropriate adjustment of the cone voltage was used to determine bioflavonoid content of five citrus species (tangerine, sanguine, sour orange, lemon and grapefruit) cultivated in Greece which come from citrus varieties analyzed for the first time. The main groups of bioflavonoids found in the juice of the citrus species according to HPLC retention times, spectral data and literature references were O-glycosylated flavanones and flavones, C-glucosylated flavones, O-glucosylated flavones, O-C-glucosylated flavones like saponarin and a phenolic derivative.
采用高效液相色谱法，通过紫外可见光电二极管阵列检测器-正电喷雾电离质谱联用技术[HPLC-UV-vis-DAD-(+ESI)-MS]，并适当调整锥孔电压以增强碎片离子，来测定希腊种植的五个柑橘品种（蜜橘、血橙、酸橙、柠檬和葡萄柚）的生物类黄酮含量，这些柑橘品种来自首次分析的柑橘变种。根据高效液相色谱保留时间、光谱数据和文献参考，在柑橘品种果汁中发现的主要生物类黄酮组分为O-糖基化黄烷酮和黄酮、C-葡萄糖基化黄酮、O-葡萄糖基化黄酮、O-C-葡萄糖基化黄酮（如皂草苷）和一种酚类衍生物。
